Phase transition-controlled flip-flop in asymmetric lipid membranes

The Journal of Physical Chemistry. B
Yujia JingSofia Svedhem

Abstract

Lipid membrane asymmetry is of fundamental importance for biological systems and also provides an attractive means for molecular control over biomaterial surface properties (including drug carriers). In particular, temperature-dependent changes of surface properties can be achieved by taking advantage of distinct phase transitions in lipid membrane coatings where lipids exchange (flip-flop) between leaflets. In this study, temperature is used to control flip-flop of lipids in asymmetric lipid membranes on planar solid supports, where the two leaflets of the lipid membrane are in different phase states. More specifically, the lower leaflet is prepared from a supported lipid membrane composed of a high Tm lipid mixture of phosphocholine (PC), phosphatidylserine (PS), and a bioactive lipid on TiO2, followed by selective removal of the top leaflet by detergent. Next, at a lower temperature, where the remaining leaflet is in the gel state, a top leaflet of a different lipid composition and in the fluid phase is formed. Phase transition-induced changes in membrane surface properties following upon temperature-activation of the prepared asymmetric membrane are demonstrated by the detection of biotinylated lipids, which were initially ...Continue Reading

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Citations

Jan 20, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Hannah K Wayment-SteeleAngelika Kunze
Jul 17, 2015·Journal of Applied Toxicology : JAT·Baoyong ShaFeng Xu
Mar 29, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Xian KongZheng Liu
Mar 31, 2015·The Journal of Physical Chemistry. B·P Losada-PérezP Wagner

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