Phase variants of Bordetella bronchiseptica arise by spontaneous deletions in the vir locus

Molecular Microbiology
D M MonackS Falkow

Abstract

Bordetella bronchiseptica is a common respiratory tract pathogen of many mammalian species. Nucleotide sequences from the locus involved in coordinate regulation of B. pertussis virulence factors, vir, were shown to have a high degree of homology to chromosomal DNA from virulent (Vir+) and avirulent (Vir-) strains of B. bronchiseptica. Small deletions, 50 bp to 500 bp, within the vir locus were found in some of the Vir- phase variants. The vir locus and the adjacent 5' portion of the fhaB structural gene were cloned from the parental Vir+ B. bronchiseptica strain on a 23.5 kb BamHI fragment. Restriction enzyme mapping of the cloned B. bronchiseptica vir locus revealed similarities with and differences from the previously cloned B. pertussis vir locus. The cloned B. bronchiseptica vir locus complemented spontaneous Vir- variants of Bordetella pertussis and B. bronchiseptica as well as vir::Tn5 mutants of B. pertussis. Comparison of various functions of the vir loci of B. bronchiseptica and B. pertussis revealed some interesting differences in the coordinate regulation of virulence factors.

References

Sep 1, 1979·Infection and Immunity·J L CowellC R Manclark
Feb 1, 1989·The Journal of Infectious Diseases·D M MonackS Falkow
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D A RelmanS Falkow
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B AricòR Rappuoli
Feb 1, 1986·Infection and Immunity·S W LeeE G Osen
Nov 1, 1987·Molecular Microbiology·B AricòR Rappuoli
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R Gross, R Rappuoli
Jun 1, 1987·Microbial Pathogenesis·F R MooiW Gaastra
Mar 1, 1987·Journal of Clinical Microbiology·C J PapasianG R Hodges
Apr 1, 1985·Journal of General Microbiology·A J Lax
May 1, 1980·Plasmid·P Starlinger
Jun 1, 1982·Journal of Clinical Microbiology·D A Bemis, B J Plotkin
Jul 1, 1980·Cell·M P Calos, J H Miller
Apr 1, 1981·The Journal of Infectious Diseases·J W EzzellC R Manclark
Nov 1, 1981·Journal of Medical Microbiology·E O IdigbeA C Wardlaw
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Jan 1, 1984·Infection and Immunity·A A Weiss, S Falkow
Jun 1, 1984·Infection and Immunity·M S Peppler, M E Schrumpf
Mar 1, 1960·The Journal of Hygiene·B W LACEY

Citations

Apr 15, 2010·Medical Microbiology and Immunology·Roy GrossKarin Schmitt
Jul 30, 2008·Antonie van Leeuwenhoek·Florence Wisniewski-Dyé, Ludovic Vial
Aug 2, 2003·Research in Microbiology·Beatriz N. Passerini De RossiM A Franco
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M Leimeister-WächterT Chakraborty
Mar 29, 2000·Journal of Bacteriology·Dagmar Beier, R Frank
Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·M H YukJ F Miller
Jul 21, 2009·BMC Microbiology·Melanie LechnerRoy Gross
Dec 17, 2002·The Journal of General and Applied Microbiology·Beatriz N. Passerini De RossiM A Franco
Jan 1, 1991·Critical Reviews in Microbiology·C J LeeJ P Li
Oct 1, 1991·Molecular Microbiology·B AricòR Rappuoli
Jul 5, 2001·International Journal of Medical Microbiology : IJMM·A Bock, R Gross
Jun 15, 2007·PLoS Computational Biology·Juilee ThakarRéka Albert
Jul 1, 1995·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·M W JackwoodP D Cox
Sep 18, 2001·Letters in Applied Microbiology·L E FriedmanM A Franco
Oct 20, 2005·Environmental Microbiology·Daan van den BroekBen Lugtenberg

Related Concepts

Genus Bordetella
Bordetella pertussis Bacterium
Partial Monosomy
Chromosomes
Cis-Trans Test
Exohemagglutinins
Lac Gene
Early Promoters, Genetic
Variation (Genetics)
Pathogenicity

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.