PHBV/PLA/Col-Based Nanofibrous Scaffolds Promote Recovery of Locomotor Function by Decreasing Reactive Astrogliosis in a Hemisection Spinal Cord Injury Rat Model

Journal of Biomedical Nanotechnology
Tengfei ZhaoQixin Chen

Abstract

Biomaterials are used to aid in the regeneration of damaged tissue and in promotion of axonal guidance following spinal cord injury (SCI). In the present study, electrospun composite poly(hydroxybutyrate-cohydroxyvalerate) (PHBV), poly(lactic acid) (PLA), and collagen (Col) nanofibrous scaffolds were applied to determine their roles in neural regeneration and recovery in a rat model of SCI. The morphological and chemical properties of the electrospun scaffolds were investigated. The growth and proliferation of astrocytes on the scaffolds were assessed by MTT assay. The differentiation and gene expression of astrocytes on the scaffolds were measured by immunofluorescence and quantitative real-time polymerase chain reaction (q-PCR) assays. In a rat spinal cord hemisection model with 3-mm defects, 80 Sprague-Dawley rats were randomly divided into five groups: Sham group, SCI group, SCI+PHBV/PLA group, SCI+PHBV/PLA/Col (70:30) group, and SCI+PHBV/PLA/Col (50:50) group. The Basso-Beattie-Bresnahan (BBB) scores were evaluated every week postsurgery, and (immuno) histological and protein analyses were performed on specimens at 8 weeks. PHBV/PLA/Col scaffolds strongly inhibited the activation of astrocytes without decreasing their prol...Continue Reading

Citations

Jul 21, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tengfei ZhaoQixin Chen
Feb 20, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tengfei ZhaoKan Xu
Jan 27, 2021·Neurochemistry International·Hun-Jin JeongSo-Jung Gwak
Dec 16, 2020·ACS Biomaterials Science & Engineering·Walaa A AbbasNageh K Allam
Jan 16, 2021·ACS Biomaterials Science & Engineering·Zhe GongChengzhen Liang

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