Phe-met-arg-phe (FMRF)-amide is a substrate source of NO synthase in the gastropod nervous system

Cell and Tissue Research
T RoszerG Bánfalvi

Abstract

The possible involvement of the L-arginine-containing Phe-met-arg-phe (FMRF)-amide (FMRFa) in neuronal nitric oxide (NO) biosynthesis was studied in a gastropod species. We found NADPH-diaphorase-positive neurons and FMRFa-containing fibers in close proximity in the enteric nervous system. Administration of L-arginine and FMRFa induced quantitatively similar nitrite production in both intact intestinal tissues and tissue homogenates. These changes could be prevented by the presence of NOARG (an NO synthase inhibitor). Neither chemically modified FMRFa (D-arginine instead of L-arginine) nor amino acid constituents of FMRFa (methionine, phenylalanine) affected basal nitrite production. FMRFa-induced alterations were reduced in the presence of Na+ channel blockers (tetrodotoxin, amiloride, lidocaine), the Na+/K+ATPase inhibitor ouabain, or protease inhibitors (leupeptine, pepstatine-a). FMRFa and its amino acid constituents were analyzed by paper chromatography. When FMRFa was added to tissue homogenates, the peptide was eliminated within 1-2 min, whereas methionine, phenylalanine, arginine, and citrulline levels were elevated simultaneously. We tested the effects of FMRFa, L-arginine, and NOARG on intestinal contractile activity....Continue Reading

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Citations

Mar 4, 2009·Cell and Tissue Research·Tamás RoszerGáspár Bánfalvi
Aug 7, 2014·Cell and Tissue Research·Tamás Rőszer, Éva D Kiss-Tóth
Feb 5, 2008·Peptides·Estuardo López-VeraEdgar P Heimer de la Cotera
Apr 29, 2011·Peptides·Tamás Rőszer, Gáspár Bánfalvi
Apr 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yang ZhuChang-Feng Chi

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