Phellinus linteus polysaccharide extracts increase the mitochondrial membrane potential and cause apoptotic death of THP-1 monocytes

Chinese Medicine
Leo Jld van Griensven, Harrie A Verhoeven

Abstract

The differentiation resp. death of human monocytic THP-1 cells induced by polysaccharide extracts of the medicinal mushrooms Phellinus linteus, Agaricus bisporus and Agaricus brasiliensis have been studied. This study aims to identify leads for the causal effects of these mushroom components on cell differentiation and death. THP-1 cells were treated with different polysaccharide extracts of mushrooms and controls. Morphological effects were observed by light microscopy. Flow cytometry was applied to follow the cell differentiation by cell cycle shifts after staining with propidium iodide, changes of mitochondrial membrane potential after incubation with JC-1, and occurrence of intracellular reactive oxygen species after incubation with hydroethidine. Principal component analysis of the data was performed to evaluate the cellular effects of the different treatments. P. linteus polysaccharide extracts induced dose-dependent apoptosis of THP-1 cells within 24 h, while A. bisporus and A. brasiliensis polysaccharide extracts caused differentiation into macrophages. A pure P. linteus polysaccharide had no effect. Apoptosis was inhibited by preincubating THP-1 cells with human serum. The principal component analysis revealed that P. ...Continue Reading

Citations

Oct 31, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maja KozarskiLeo van Griensven
Dec 2, 2015·The American Journal of Chinese Medicine·Chien-Kuo HanYuan-Li Huang
May 20, 2015·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Xiao-hui LiHong-min Wang
May 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wenhua ChenLingchuan Xu
Jul 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Youngin KwonYoung-Ho Lee
Mar 20, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fei-Fei WangNing Li

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis