Phenazine methosulfate stimulation of ouabain-sensitive Rb+ uptake by HeLa cells: effects of respiratory inhibitors, anaerobiosis, and ascorbate

Journal of Cellular Biochemistry
T IkeharaH Miyamoto


phenazine methosulfate (PMS) stimulates ouabain-sensitive Rb+ uptake by HeLa cells. This stimulation cannot be attributed to the effect of the dye on the intracellular Na+ or ATP content. Respiratory inhibitors, such as 5 mM NaCN and 5 microM rotenone, and anaerobic conditions enhance the stimulation of Rb+ uptake by PMS. Cellular respiration is stimulated, but lactate production is reduced in the presence of PMS, irrespective of the presence of respiratory inhibitors. Cellular NADH is oxidized markedly on addition of PMS plus inhibitors, but it is not affected by addition of the inhibitors only. In the presence of a high concentration of PMS, PMS-stimulated ouabain-sensitive Rb+ uptake is inhibited by addition of ascorbate. From these results it is concluded that Na+K-pump activity is closely related to the cellular redox state.


Jan 1, 1977·Journal of Supramolecular Structure·H N Christensen
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J Garcia-SanchoH N Christensen
Jun 1, 1973·Analytical Biochemistry·C Bernofsky, M Swan
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Jan 1, 1982·The Japanese Journal of Physiology·T IkeharaK Kaniike
Sep 1, 1954·Biochimica Et Biophysica Acta·T P SINGER, E B KEARNEY

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