Phenethyl isothiocyanate inhibits colorectal cancer stem cells by suppressing Wnt/β-catenin pathway

Phytotherapy Research : PTR
Yue ChenHong-Yu Han

Abstract

Cancer stem cells (CSCs) are considered to play essential roles in the process of origination, proliferation, migration, and invasion of cancer, and their properties are regulated by Wnt/β-catenin pathway. Phenethyl isothiocyanate (PEITC) is a natural product obtained from cruciferous vegetables with anticancer activities. The present study aimed to investigate the inhibitory effect and the underlying mechanisms of PEITC on colorectal CSCs. In this study, we found that PEITC can significantly reduce the size and number of colorectal cancer cell spheroids in serum-free medium. With increasing PEITC concentrations (10-40 μM), the number of spheroids was reduced to about 10% of the control group, and the percentage of CD133+ cells was decreased by about 3-16 folds. PEITC also decreased the expression of CSC markers. Meanwhile, inhibition of proliferation as well as induction of apoptosis of colorectal CSCs was observed after PEITC treatment. Furthermore, through activating Wnt/β-catenin pathway with LiCl, the inhibitory effects of PEITC on colorectal CSCs were diminished. Our data suggested that PEITC can be an effective inhibitor of colorectal CSCs by targeting Wnt/β-catenin pathway.

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Citations

Mar 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alena LiskovaDietrich Büsselberg
Jul 26, 2019·Phytotherapy Research : PTR·Qi ZhangHong-Yu Han
Jul 11, 2020·Environmental Toxicology·Taotao ZhangCaiyun Zhong
Jul 23, 2020·Frontiers in Pharmacology·Wen-Kai YuJiang-Jiang Qin
Jan 13, 2021·Seminars in Cancer Biology·Madhumitha Kedhari SundaramArif Hussain
Jan 5, 2021·Biotechnology and Applied Biochemistry·Zhengxin WuXuehui Hong
Mar 6, 2021·Phytotherapy Research : PTR·Bahare SalehiMiquel Martorell
Feb 25, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Ji Min ShinChu Won Nho
Jul 18, 2021·Seminars in Cancer Biology·Man ChuZhi-Wei Wang
Jul 30, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jian XiaoYan Zhang
Nov 19, 2021·Cancer Communications·Valdenizia R SilvaDaniel P Bezerra
Jan 9, 2022·Journal of Cellular and Molecular Medicine·Xiaobo NieWei-Dong Chen

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