Phenethyl isothiocyanate (PEITC) inhibits growth of ovarian cancer cells by inducing apoptosis: role of caspase and MAPK activation

Gynecologic Oncology
K S SatyanLaurent Brard

Abstract

Epithelial ovarian cancer has the highest mortality rate among gynecologic cancers. Chemotherapy is an essential component of its treatment. While isothiocyanates are known to possess chemopreventive effects against various cancers, yet little is known about their chemotherapeutic potential in ovarian cancer (OC). In the present study, we examined the antiproliferative and apoptotic effect of phenethyl isothiocyanate (PEITC), a naturally occurring isothiocyanate on OVCAR-3 cells. Cytotoxic activity of PEITC on OVCAR-3 cells was determined using cell proliferation, apoptosis (DNA fragmentation and TUNEL assay) and caspase-activation studies. The role of PARP-1, Bax, and Bcl-2 in apoptosis was analyzed by Western blotting. Activation of JNK1/2, p38, Akt, ERK1/2, and c-Myc was examined by immunoblotting. Specific inhibitors of caspases, JNK1/2, p38, and MEK were used to corroborate these data. PEITC was cytotoxic to OVCAR-3 cells, and inhibited proliferation in a dose-dependent fashion (IC(50) = 23.2 microM). PEITC induced apoptosis by activating caspase-3 and -9, without capsase-8 activation. Anti-apoptotic Bcl-2 levels were suppressed while pro-apoptotic Bax levels were enhanced. PEITC suppressed activation of Akt, ERK1/2, and t...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Sep 1, 1996·American Journal of Obstetrics and Gynecology·G S RoseJ C Hiserodt
Oct 6, 1997·Genes & Development·Z Q WangE F Wagner
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J M JürgensmeierJ C Reed
Aug 28, 1998·Science·A Ashkenazi, V M Dixit
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Jul 7, 2000·Seminars in Surgical Oncology·C H Holschneider, J S Berek
Jan 4, 2001·Drug Metabolism Reviews·S S Hecht
Mar 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Robert E BristowF J Montz
May 2, 2002·Anti-cancer Drugs·Paul J Thornalley
Oct 18, 2002·Expert Review of Anticancer Therapy·Ryan A Dick, Thomas W Kensler
Feb 21, 2003·International Journal of Cancer. Journal International Du Cancer·Keiran S M Smalley
Aug 29, 2003·Cellular and Molecular Life Sciences : CMLS·P RoseC N Ong
Nov 9, 2004·The Journal of Clinical Endocrinology and Metabolism·Jung-Hye ChoiPeter C K Leung
Sep 22, 2005·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·C-H ChenS-N Chow
Sep 24, 2005·Pharmaceutical Research·Yan JiMarilyn E Morris

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Citations

Oct 1, 2011·Chinese Journal of Cancer·Jun-Lin LiXia Fan
Aug 6, 2011·Natural Product Research·Hongxia ChenXijuan Chen
Nov 10, 2015·Cell Biochemistry and Function·Nada H EisaMamdouh M El-Shishtawy
Jan 30, 2016·Clinical Oral Investigations·Cheng-Chang YehYi-Ting Deng
Jul 1, 2011·Gynecologic Oncology·Xiangdong MaDetang Wang
Jun 6, 2009·Pigment Cell & Melanoma Research·Subbarao V Madhunapantula, Gavin P Robertson
Jan 27, 2009·Environmental and Molecular Mutagenesis·Carmela FimognariPatrizia Hrelia
May 20, 2011·Molecular Nutrition & Food Research·Xiantao WangFung-Lung Chung
Aug 21, 2013·Cell Biology International·Tao TangWen-Yue Wang
Jul 12, 2012·Journal of Ovarian Research·Daniel K Chan, W Keith Miskimins
May 27, 2015·Indian Journal of Pharmaceutical Sciences·Prachi BansalM M Srivastava
Aug 1, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Andrzej PawlikAlina Grzanka
Nov 2, 2012·Cancer Biology & Therapy·Nilotpal RoyPradip Raychaudhuri
Jun 28, 2011·Biochimica Et Biophysica Acta·Kristin K Brown, Mark B Hampton
Jul 16, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Anna PtakEwa L Gregoraszczuk
Aug 20, 2015·PloS One·Abdulrahman Khazim Al-AsmariMozaffarul Islam
Mar 9, 2010·Bioscience, Biotechnology, and Biochemistry·Ichiro ShirasugiMasahito Suiko
Sep 1, 2016·Frontiers in Nutrition·Francisco J BarbaMohamed Koubaa
Sep 8, 2017·International Journal of Molecular Sciences·Yi Liu, Moul Dey
Sep 14, 2017·Bioscience, Biotechnology, and Biochemistry·Moe NagamiTakashi Nagasawa
Oct 5, 2018·The American Journal of Chinese Medicine·Hung-En ChenThomas I-Sheng Hwang
Dec 15, 2007·Molecular Nutrition & Food Research·Min-Hsiung PanChi-Tang Ho
May 7, 2009·Acta Pharmacologica Sinica·Xiang WuKe Xu
Oct 4, 2019·Antioxidants & Redox Signaling·Hsin Yao ChiuReshma Taneja
Feb 14, 2020·Anti-cancer Agents in Medicinal Chemistry·Kanchana UsuwanthimThitiya Luetragoon
Jun 29, 2018·International Journal of Molecular Sciences·Sofia C Nunes, Jacinta Serpa
Oct 8, 2019·Antioxidants & Redox Signaling·Alma MartelliVincenzo Calderone

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