PMID: 18198396Jan 17, 2008Paper

Phenethyl isothiocyanate suppresses receptor activator of NF-kappaB ligand (RANKL)-induced osteoclastogenesis by blocking activation of ERK1/2 and p38 MAPK in RAW264.7 macrophages

BioFactors
Akira MurakamiHajime Ohigashi

Abstract

Osteoclastogenesis is induced by differentiation of hemopoietic cells of monocyte-macrophage lineage into bone-resorbing osteoclasts. The process is initiated by receptor activator of NF-kappaB ligand (RANKL) and resultant activation of mitogen-activated protein kinases (MAPK), including extracellular signal-regulated kinase (ERK)1/2, as well as the NFkappaB pathway. Phenethyl isothiocyanate (PEITC), a phytochemical present in various cruciferous plants, has been shown to disrupt those signaling pathways in several cell types. In this study, we examined the efficacy of PEITC for suppressing RANKL-induced osteoclastogenesis in RAW264.7 murine macrophages and addressed the underlying molecular mechanisms. PEITC (2-10 microM) suppressed osteoclastogenesis in a concentration dependent manner, as detected by tartarate-resistant acid phosphatase (TRAP) activity and microscopic observations. RANKL-up-regulated extracellular signal-regulated kinase (ERK)1/2 and p38 mitogen-activated protein kinase (MAPK) activities were attenuated by PEITC, whereas c-Jun N-terminal kinase (JNK1/2) activation was increased. PEITC also abrogated the RANKL-induced degradation of IkappaB-alpha, a suppressive partner of nuclear factor kappaB (NFkappaB), the...Continue Reading

References

Jan 4, 1993·Journal of Immunological Methods·D SladowskiM Balls
Sep 12, 2002·Arthritis Research·Nobuyuki UdagawaTatsuo Suda
Sep 19, 2002·The Journal of Biological Chemistry·Hitoshi HotokezakaKoji Nakayama
Mar 10, 2004·International Review of Neurobiology·Svetlana V Kyosseva
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alok C BhartiBharat B Aggarwal
May 19, 2004·FEBS Letters·Jeong Hwan JeHae Young Chung
May 27, 2004·Natural Product Reports·Birgit Holst, Gary Williamson
Jul 29, 2004·JAMA : the Journal of the American Medical Association·Lorenz C Hofbauer, Michael Schoppet
Nov 24, 2004·Drug Metabolism Reviews·Franca Bianchini, Harri Vainio
May 26, 2005·Cancer Letters·Jeong-Sang Lee, Young-Joon Surh
Nov 30, 2005·Immunological Reviews·Lori A Ehrlich, G David Roodman
May 17, 2006·Journal of Lipid Research·Md Mizanur RahmanGabriel Fernandes
Jun 30, 2006·Cellular Signalling·Laura CallerosAntonio Chiloeches
Jul 11, 2006·BioFactors·Yoshimasa Nakamura, Noriyuki Miyoshi
Oct 21, 2006·Biochemical and Biophysical Research Communications·Hao HuangHong-Hee Kim

❮ Previous
Next ❯

Citations

Nov 16, 2011·Molecular Nutrition & Food Research·Min-Hsiung PanChi-Tang Ho
Jan 26, 2011·Expert Opinion on Therapeutic Targets·Constance Lay Lay Saw, Ah-Ng Tony Kong
Nov 12, 2014·Chemical Research in Toxicology·Sarandeep S S BoyanapalliAh-Ng Tony Kong
Jun 22, 2019·Journal of Cellular Physiology·Yuan XiaoLingbo Kong
Jun 6, 2009·Journal of Agricultural and Food Chemistry·Min-Hsiung PanChi-Tang Ho

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.