Phenobarbital-inducible gene expression in developing rat liver: relationship to hepatocyte function.

Biochimica Et Biophysica Acta
S Marie, T Cresteil

Abstract

The expression of phenobarbital-, pregnenolone 16 alpha-carbonitrile- and polycyclic aromatic hydrocarbon-inducible cytochromes P-450 and of phenobarbital-inducible UDP-glucuronosyltransferase was examined in developing rat liver. RNAs coding for these proteins were present in fetal rat liver and their respective concentrations remained quite stable in non-induced animals. Inducers differently affected the concentration of RNAs: clofibrate had no action, whereas methylcholanthrene was highly active in fetal liver. Induction by phenobarbital gradually increased during ontogenesis, in parallel with the augmentation of the number of hepatocyte cells in the liver. Our contribution definitively demonstrates that the ability of phenobarbital to enhance P-450 and UDPGT RNAs is strictly restricted to hepatocytes and remains roughly unchanged throughout ontogenesis. In addition, phenobarbital was also able to potentiate the inducing capacity of methylcholanthrene (i.e., raising the TCDD-binding protein) exclusively in hepatocytes. This is the first direct evidence that the number of hepatocytes in the liver, rather than a biochemical maturation, controls the expression of phenobarbital-inducible genes. Pregnenolone 16 alpha-carbonitrile...Continue Reading

References

Mar 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J ChianaleJ J Gumucio
Nov 26, 1986·Biochemical and Biophysical Research Communications·K T ShiverickM O James
Jan 1, 1971·Biology of the Neonate·J C LarrocheM Bennoun
Jun 1, 1968·The Biochemical Journal·P G Holt, I T Oliver
Jun 15, 1982·Biochemical and Biophysical Research Communications·T CresteilJ P Leroux

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Citations

Oct 11, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Steven N HartXiao-bo Zhong
Sep 5, 2002·Seminars in Neonatology : SN·Phyllis A Dennery
Dec 31, 1997·Microscopy Research and Technique·K J Rich, A R Boobis
Oct 27, 2015·PloS One·Sumedha S GunewardenaJulia Yue Cui
Feb 21, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Nichole R Vansell, Curtis D Klaassen

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