Phenol- and toluene-degrading microbial populations from an aquifer in which successful trichloroethene cometabolism occurred.

Applied and Environmental Microbiology
M R FriesJ M Tiedje

Abstract

We characterized the bacterial populations that grew in a Moffett Field, Calif., aquifer following three sequential field tests of phenol- or toluene-driven cometabolism of trichloroethene (TCE). Reducing the toluene and phenol concentrations in most-probable-number (MPN) tubes from 50 to 5 ppm increased the population density measured for these degraders by 1.5 and 1 log units, respectively, suggesting that natural populations might be quite sensitive to these substrates. Phenol and toluene degraders were isolated from the terminal MPN dilution tubes; 63 genetically distinct strains were identified among the 273 phenol- and toluene-degrading isolates obtained. TCE was cometabolized by 60% of the genetically distinct strains. Most strains (57%) grew on both phenol and toluene, and 78% of these strains hybridized to the toluene ortho-monooxygenase (TOM) probe. None of the strains hybridized to probes from the four other toluene oxygenase pathways. Gram-positive strains comprised 30% of the collection; all of these grew on phenol, and 47% of them also grew on toluene, but none hybridized to the TOM probe. Among the gram-negative strains, 86% of those that grew on both toluene and phenol hybridized to the TOM probe, while only 5% ...Continue Reading

References

May 1, 1990·Applied and Environmental Microbiology·B R FolsomP H Pritchard
Jul 1, 1988·Applied and Environmental Microbiology·L P Wackett, D T Gibson
May 1, 1987·Applied and Environmental Microbiology·M J NelsonP H Pritchard
Sep 1, 1969·The Journal of Applied Bacteriology·J D Owens, R M Keddie
Jun 1, 1984·Applied and Environmental Microbiology·S Simkins, M Alexander
Apr 1, 1995·Applied and Environmental Microbiology·M S ShieldsC Somerville
Dec 1, 1994·Biodegradation·J Powlowski, V Shingler
Aug 1, 1994·Applied and Environmental Microbiology·M R FriesJ M Tiedje
Mar 1, 1996·Applied and Environmental Microbiology·J G LeahyR H Olsen
Jun 1, 1989·Applied and Environmental Microbiology·M S ShieldsP H Pritchard
Mar 1, 1996·Applied and Environmental Microbiology·A E MarsD B Janssen

❮ Previous
Next ❯

Citations

May 30, 2013·Applied Microbiology and Biotechnology·Kyunghwa BaekKlaus Nüsslein
Jun 18, 2005·Letters in Applied Microbiology·L A O'Sullivan, E Mahenthiralingam
Mar 4, 2008·FEMS Microbiology Letters·Silvia TabacchioniRenato Fani
Jul 24, 2010·Journal of Medical Microbiology·Maria Cristiana PapaleoPeter Vandamme
Jun 1, 2011·Environmental Microbiology Reports·Nicholas V ColemanAndrew J Holmes
Jun 1, 2008·Indian Journal of Microbiology·Pooja SharmaRup Lal
Dec 31, 2002·Folia Microbiologica·J RůzickaM Nĕmec
Nov 30, 2018·Microbiology and Molecular Biology Reviews : MMBR·Ashish GuptaAnne Grove
Apr 6, 2017·Environmental Science and Pollution Research International·Iva DolinováAlena Ševců
Oct 9, 2001·Journal of Contaminant Hydrology·J ParkL M Abriola
Dec 14, 2005·Applied and Environmental Microbiology·A E MarsD B Janssen
Jul 1, 1997·Applied and Environmental Microbiology·V G MathesonL J Forney

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.