Oct 28, 1980

Phenol hydroxylase from yeast: a lysyl residue essential for binding of reduced nicotinamide adenine dinucleotide phosphate

Biochemistry
H Y Neujahr, K G Kjellén

Abstract

The inducible enzyme phenol hydroxylase from Trichosporon cutaneum is a FAD-containing monooxygenase which catalyzes the NADPH-dependent hydroxylation of phenol to catechol. The enzyme contains 16 cysteinyl residues, 6--8 of which are essential for retention of FAD and for activity. The complete amino acid composition is now reported as well as the results of studies with amino group reagents. A number of amino group reagents inhibit the enzyme severely, most of them with a concomitant, more or less extensive release of FAD. P-pyridoxal inhibits the enzyme specifically, without affecting its FAD content. The P-pyridoxal modified enzyme has a characteristic absorption peak at 325 nm indicating the presence of a N epsilon-pyridoxyllysyl derivative. Such a derivative was identified in hydrolysates of the modified enzyme by means of column chromatography. The results obtained with P-pyridoxal-modified enzyme indicate that a lysyl residue is essential for activity by being involved in binding of the co-substrate NADPH. These results are corroborated by kinetic studies showing competition between P-pyridoxal and NADPH for the binding site. The reactivity of the essential lysyl residue toward P-pyridoxal is significantly increased in ...Continue Reading

  • References11
  • Citations17

References

Mentioned in this Paper

Phenol 2-monooxygenase
Phenol
Pyridoxal Phosphate
Ligand Binding Domain
Trichosporon cutaneum allergenic extract
Catechols
Metabolic Inhibition
Phenols
Spectrophotometry
NADP

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