Phenolic Extraction of Moringa Oleifera Leaves Induces Caspase-Dependent and Caspase-Independent Apoptosis through the Generation of Reactive Oxygen Species and the Activation of Intrinsic Mitochondrial Pathway in Human Melanoma Cells.

Nutrition and Cancer
Bich Hang DoChinh Chung Doan

Abstract

Moringa oleifera Lam. has long been used to treat many diseases, including diabetes, aging, inflammatory, and cancer. Many studies have revealed that the crude extract of Moringa oleifera Lam. leaves possesses anticancer property. Therefore, in this study, the extract of Moringa oleifera leaves was fractionated using different solvents to figure out the most effective fraction for anti-proliferative effect on melanoma cells. Methanol extract (MO-ME), hexane fraction (MO-HE), chloroform fraction (MO-CH), ethyl acetate fraction (MO-EA), and water-soluble fraction (MO-WA) of Moringa oleifera leaves were prepared. Total phenolic and flavonoid contents were determined. The anti-proliferative activity on melanoma cells and normal cells was investigated using WST-1 assay. The apoptotic activity was assessed by testing DNA condensation, DNA fragmentation, and phosphatidylserine (PS) externalization. The expression of apoptosis-related genes, the mitochondrial depolarization, and reactive oxygen species (ROS) were then examined to clarify the underlying molecular mechanisms. In this regard, MO-ME, MO-EA, and MO-CH inhibited the proliferation of both A375 human melanoma cells and A2058 human melanoma cells, but had little effect on WS1 n...Continue Reading

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Citations

Jul 13, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Nguyen Hoai NguyenBich Hang Do
Aug 14, 2021·Journal of Toxicology and Environmental Health. Part a·Doan Chinh ChungNghia Son Hoang

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Methods Mentioned

BETA
PCR
electrophoresis
flow cytometry
Reverse Transcription PCR
protein assay
FACS
nuclear translocation

Software Mentioned

EA
MO
CellQuest Pro
Image J

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