PMID: 333Nov 1, 1975

Phenomenon of hot-cold hemolysis: chelator-induced lysis of sphingomyelinase-treated erythrocytes

Infection and Immunity
C J SmythT Wadström

Abstract

Staphylococcus aureus produces a phospholipase C specific for sphingomyelin (beta-hemolysin). Erythrocytes with approximately 50% sphingomyelin in their membranes, e.g., from sheep, have been shown to have up to 60% of this phospholipid hydrolyzed by this enzyme at 37 C in isotonic buffered saline without hemolysis. Cooling of sphingomyelinase C-treated erythrocytes to 4 C causes complete lysis of the cells, a phenomenon known as hot-cold hemolysis. The addition of ethylenediaminetetraacetate (EDTA) to sheep erythrocytes preincubated with sphingomyelinase C was found to induce rapid hemolysis at 37 C. The treated cells became susceptible to chelator-induced hemolysis and to hot-cold hemolysis simultaneously, and the degree of lysis of both mechanisms increased equally with prolonged preincubation with sphingomyelinase C. Erythrocytes of species not readily susceptible to hot-cold hemolysis were equally insusceptible to chelator-induced lysis. Chelators of the EDTA series were the most effective, whereas chelators more specific for Ca2+, Zn2+, Fe2+, Cu2+, and Mg2+ were without effect. The rate of chelator-induced lysis was dependent on the preincubation period with beta-hemolysin and on the concentration of chelator added. The o...Continue Reading

Citations

Apr 18, 2016·Current Microbiology·Wioletta KmieciakMarcin Ciszewski
Jan 1, 1983·Transactions of the New York Academy of Sciences·A W Bernheimer
Oct 1, 1981·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·T MalmqvistR Möllby

Related Concepts

Metal Antagonists
Pentetates
Edetic Acid, Sodium Salt
Erythrocytes
Hemolysin
Hemolysis
Osmolality
Phenanthrolines
Phosphoric diester hydrolase
Dall Sheep

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