Phenotype and function of stromal cells cloned from the ileal Peyer's patch of sheep

Stem Cells
Philip GriebelG Ferrari

Abstract

The ileal Peyer's patch (PP) is the major site of B cell production and immunoglobulin diversification in lambs, but the factors which regulate these processes are poorly understood. As a first step toward identifying possible regulatory mechanisms, stable long-term cultures of ileal PP stromal cells were established at the clonal level. Four distinct cell types were identified by their phenotype and growth requirements. Immunohistochemical staining confirmed that all clones were mesenchymal (vimentin+; cytokeratin-) in origin and were negative for T cell, B cell, and macrophage markers. Three cell lines were negative for major histocompatibility complex (MHC) I and II molecules, but one cell line, SCN, expressed MHC I, MHC II and CD44 molecules, and a subpopulation of SCN cells expressed BAQ44A, a B cell differentiation molecule. The four cell lines produced different types and amounts of extracellular matrix proteins, and their growth was not influenced by exogenous human interleukin 1 (IL-1), IL-2, transforming growth factor beta 1 (TGF beta 1), or bovine fibroblast growth factor (FGF) but was influenced by serum. When tested for their capacity to support lymphocyte growth, all clones produced a soluble factor(s) that was mi...Continue Reading

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Citations

Mar 6, 2003·Molecular Immunology·Neetu Gupta-RossiAuriel Dahan
Jul 31, 1998·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M KedingerP Simon-Assmann
Feb 3, 1999·Annals of the New York Academy of Sciences·M KedingerJ N Freund
Jan 1, 1997·International Reviews of Immunology·J Reynolds
Mar 31, 2004·Cellular Immunology·Anil K NichaniPhilip J Griebel

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