PMID: 9438908Jan 24, 1998Paper

Phenotype and proliferation characteristics of cultured spindle-shaped cells obtained from normal human skin and lesions of dermatofibroma, Kaposi's sarcoma, and dermatofibrosarcoma protuberans: a comparison with fibroblast and endothelial cells of the dermis

Journal of Dermatological Science
B K BonishB J Nickoloff

Abstract

Normal human dermis contains mesenchymal cells that are generally referred to as fibroblasts. However the relationships between fibroblasts and endothelial cells with respect to the types of spindle-shaped cells that are present in cultures obtained from tumor bearing-skin is unclear. To explore the potential heterogeneity amongst dermal-derived cells that grow in culture with a spindle-shaped morphology, we compared the immunophenotype and growth characteristics of several types of cells. Besides dermal fibroblasts and microvascular endothelial cells derived from normal adult skin, we also studied large vessel-derived endothelial cells, and spindle-shaped cells derived from three different tumor-bearing dermal-based neoplasms. Kaposi's sarcoma (KS), dermatofibroma (DF), and dermatofibrosarcoma protuberans (DFSP). A broad panel of eight different antibodies were used to immunophenotype the multi-passaged cultured cells. Spindle-shaped cells from all three neoplasms could be distinguished from the normal skin derived fibroblasts by their constitutive expression of factor XIIIa, and the gamma-interferon induced expression of VCAM-1. All seven types of cultured cells stained positive for s-actin and proline-4-hydroxylase, and none...Continue Reading

References

Aug 1, 1992·The British Journal of Dermatology·S AibaH Tagami
May 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·O Kocher, J A Madri
Jun 15, 1984·European Journal of Biochemistry·M HöyhtyäK Tryggvason
Apr 1, 1993·Journal of Cutaneous Pathology·D A AltmanD P Fivenson
Feb 1, 1993·Histopathology·E K DerrickD M Macdonald
Feb 1, 1996·Clinical Immunology and Immunopathology·K E ForemanB J Nickoloff
Jan 1, 1996·Anatomy and Embryology·N S GibranK A Holbrook

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Citations

Jun 8, 2001·Journal of Dermatological Science·K E ForemanB J Nickoloff
Oct 28, 1998·The American Journal of Dermatopathology·J S Silverman, A Tamsen
Apr 8, 2014·Annales de dermatologie et de vénéréologie·F BoukariJ-P Lacour

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