Phenotype characterization of pericytes during tissue repair following low-level laser therapy

Photodermatology, Photoimmunology & Photomedicine
Alena P MedradoZilton Andrade

Abstract

The action of low-level laser therapy (LLLT) on pericytes during wound healing is not well established. The objective of this study was to identify the effect of laser treatment on pericytes during tissue repair. Punch biopsies were performed on 40 Wistar rats. Twenty animals had their wounds treated with a dose of 4 J/cm(2) using a 670 nm diode laser (9 mW output, 0.031 W/cm(2)) every other day, while the controls received sham irradiation. Animals were sacrificed 3, 7, 10 and 14 days after punch biopsy. Immunohistochemistry staining with anti-desmin, anti-smooth muscle alpha-actin and anti-NG2 antibodies was used to characterize and count pericytes around blood vessels and myofibroblasts dispersed in the extracellular matrix (ECM). The morphology of pericytes was confirmed by transmission electronic microscopy. The laser group exhibited significantly more smooth muscle alpha-actin-positive staining cells at day 7 and more desmin-positive staining cells at day 10 around blood vessels. Laser treatment was also associated with higher numbers of NG2-positive staining cells, especially on days 3 and 7 post-biopsy (P<0.05). Ultrastructural findings confirmed the presence of pericytes sharing the basal membrane with endothelial cell...Continue Reading

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Citations

Oct 22, 2010·Photomedicine and Laser Surgery
Nov 10, 2016·Anais Brasileiros De Dermatologia·Ana Cristina de Oliveira GonzalezAlena Ribeiro Alves Peixoto Medrado
May 26, 2017·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Zhe Wu JinShin-Ichi Abe
Apr 17, 2019·Expert Review of Clinical Immunology·Nicholas K LarsenDevendra K Agrawal
Dec 26, 2019·Journal of Lasers in Medical Sciences·Ana Cristina GonzalezAlena R A P Medrado
Nov 8, 2020·Scientific Reports·Isabella Bittencourt do ValleIvana Márcia Alves Diniz

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