PMID: 11341997May 9, 2001Paper

Phenotype determination of anti-GM3 positive cells in atherosclerotic lesions of the human aorta. Hypothetical role of ganglioside GM3 in foam cell formation

Biochimica Et Biophysica Acta
Y V BobryshevN V Prokazova

Abstract

Earlier we reported that atherosclerotic plaques contain cells which were specifically and very intensively stained with anti-GM3 antibodies although no GM3 positive cells were detected in the normal non-diseased arterial intima. Because of their lipid inclusions, GM3 positive cells in atherosclerotic lesions seemed to be foam cells but their origin needed clarification. Using an immunohistochemical technique in the present work, we showed that some of these foam cells contained CD68 antigen. However, the most intense accumulation of GM3 occurred in the areas composed of foam cells which did not stain with any cell type-specific antibodies, including antibodies to macrophages (anti-CD68) and smooth muscle cells (anti-smooth muscle alpha-actin), perhaps, because the cell type-specific antigens were lost during the transformation of intimal cells into foam cells. Ultrastructural analysis of the areas where foam cells overexpressed GM3 demonstrated that some foam cells lacked both a basal membrane and myofilaments but contained a large number of secondary lysosomes and phagolysosomes, morphological features which might indicate their macrophage origin. Other foam cells contained a few myofilaments and fragments of basal membrane a...Continue Reading

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Citations

Dec 1, 2006·Basic Research in Cardiology·Yuri V BobryshevStella M Valenzuela
Jun 7, 2005·Atherosclerosis·Yuri V BobryshevNina V Prokazova
Aug 8, 2007·Biochemistry. Biokhimii︠a︡·E V GrachevaN V Prokazova
Apr 15, 2009·Biochemistry. Biokhimii︠a︡·N V ProkazovaN K Golovanova
Jan 9, 2014·Glycoconjugate Journal·Jin-ichi Inokuchi
May 12, 2011·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Jin-ichi Inokuchi
Dec 20, 2005·Micron : the International Research and Review Journal for Microscopy·Yuri V Bobryshev
Jan 9, 2003·Journal of Lipid Research·Peter Gargalovic, Ladislav Dory
Oct 14, 2009·FEBS Letters·Jin-ichi Inokuchi
Dec 29, 2006·Journal of Atherosclerosis and Thrombosis·Hidekatsu YanaiHitoshi Chiba
Dec 1, 2008·Obesity Research & Clinical Practice·Takashige SatoJin-Ichi Inokuchi

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