Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages

Veterinary Immunology and Immunopathology
Kenneth C McCulloughA Summerfield

Abstract

When porcine blood monocytes differentiate in vitro into macrophages, their morphology, as well as side scatter and forward scatter measured by flow cytometry, changed in a manner similar to that with human cells. During this differentiation, the initial high expression of CD molecules on porcine monocytes was down-regulated, with one exception--SWC9. Freshly isolated blood monocytes were SWC9-, but after culture the cells had become SWC9+. Thus, porcine monocytes were characterised as SWC3+SWC9-CD14high; macrophages were SWC3+SWC9+CD14low, the latter also displaying a down-regulation of CD11a/18, and, to a lesser degree, CD44. Both SWC9-CD14high monocytes and SWC9+CD14low macrophages were identifiable in freshly prepared monocytic cells from the spleen. Alveolar macrophages, on the other hand, were dominated by SWC9+CD14low cells, similar in phenotype to in vitro monocyte-derived macrophages. The consequences which these results have for studies on virus infections of porcine monocytic cells are discussed.

References

Feb 1, 1992·Scandinavian Journal of Immunology·Y RevillaE Viñuela
Feb 1, 1988·Virus Research·I C PanM Shimizu
Aug 1, 1996·Immunology Today·A Saalmüller
May 20, 1998·Veterinary Immunology and Immunopathology·J DomínguezK Burger
May 20, 1998·Veterinary Immunology and Immunopathology·J DominguezK Burger

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Citations

Aug 6, 1998·Journal of Immunological Methods·V A Natale, K C McCullough
Jan 4, 2001·Veterinary Immunology and Immunopathology·S BastaK C McCullough
May 6, 2003·Veterinary Immunology and Immunopathology·J NielsenK C McCullough
Aug 14, 1999·Immunology·S M KnoetigK C McCullough
Nov 8, 2011·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Maisam Abu-El-HaijaAliye Uc
Jul 6, 2000·Immunobiology·S ChamorroJ Domínguez
Jul 1, 2008·Developmental and Comparative Immunology·A EzquerraJ Domínguez
Nov 25, 2003·Immunology·Artur SummerfieldKenneth C McCullough
May 21, 2003·Journal of Veterinary Pharmacology and Therapeutics·F BozićI Valpotić
Jan 9, 2014·Veterinary Microbiology·Pamela LithgowDave Chapman
Jul 28, 2009·Veterinary Microbiology·Sameh BastaKenneth C McCullough
Nov 23, 2006·Genomics·Tomoko Eguchi-OgawaHirohide Uenishi
Apr 3, 2007·Veterinary Immunology and Immunopathology·Stephen M WilsonMichael Bailey
May 22, 2004·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Romeo E SanchezMaurice B Pensaert
Mar 16, 2005·Journal of Leukocyte Biology·Carole BalmelliArtur Summerfield
Jul 22, 2008·Veterinary Research·Laurence Piriou-Guzylack, Henri Salmon
Jul 19, 2013·Physics in Medicine and Biology·Antje-Christin Knopf, Antony Lomax
Feb 7, 2018·Developmental and Comparative Immunology·Carmen A SautterArtur Summerfield

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