Phenotype transitions induced by mechanical stimuli in airway smooth muscle are regulated by differential interactions of parvin isoforms with paxillin and Akt.

American Journal of Physiology. Lung Cellular and Molecular Physiology
Youliang Huang, Susan J Gunst

Abstract

Mechanical tension and humoral stimuli can induce transitions in airway smooth muscle phenotype between a synthetic inflammatory state that promotes cytokine secretion and a differentiated state that promotes the expression of smooth muscle phenotype-specific proteins. When tissues are maintained under high tension, Akt activation and eotaxin secretion are suppressed, but expression of the differentiation marker protein, smooth muscle myosin heavy chain (SmMHC), is promoted. When tissues are maintained under low tension, Akt activation and eotaxin secretion are stimulated, and the differentiated phenotype is suppressed. We hypothesized that mechanical stimuli are differentially transduced to Akt-mediated signaling pathways that regulate phenotype expression by α-parvin and β-parvin integrin-linked kinase/PINCH/parvin (IPP) signaling complexes within integrin adhesomes. High tension or ACh triggered paxillin phosphorylation and the binding of phospho-paxillin to β-parvin IPP complexes. This inhibited Akt activation and promoted SmMHC expression. Low tension or IL-4 did not elicit paxillin phosphorylation and triggered the binding of unphosphorylated paxillin to α-parvin IPP complexes, which promoted Akt activation and eotaxin se...Continue Reading

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Citations

May 21, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yidi WuSusan J Gunst
May 20, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yidi WuSusan J Gunst

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Methods Mentioned

BETA
nuclear translocation
proximity ligation
proximity ligation assay
transfection
immunoprecipitation
electrophoresis
dissection
confocal
enzymatic dissociation
ELISA

Software Mentioned

GraphPad
Duolink Images Tool

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