PMID: 8606187Apr 1, 1996Paper

Phenotypes of Bacillus subtilis mutants lacking multiple class A high-molecular-weight penicillin-binding proteins

Journal of Bacteriology
D L Popham, P Setlow

Abstract

Examination of Bacillus subtilis strains containing multiple mutations affecting the class A high-molecular-weight penicillin-binding proteins (PBPs) 1, 2c, and 4 revealed a significant degree of redundancy in the functions of these three proteins. In rich media, loss of PBPs 2c and 4 resulted in no obvious phenotype. The slight growth and cell morphology defects associated with loss of PBP 1 were exacerbated by the additional loss of PBP 4 but not PBP 2c. Loss of all three of these PBPs slowed growth even further. In minimal medium, loss of PBPs 2c and 4 resulted in a slight growth defect. The decrease in growth rate caused by loss of PBP 1 was accentuated slightly by loss of PBP 2c and greatly by loss of PBP 4. Again, a lack of all three of these PBPs resulted in the slowest growth. Loss of PBP 1 resulted in a 22% reduction in the cell radius. Cultures of a strain lacking PBP 1 also contained some cells that were significantly longer than those produced by the wild type, and some of the rod-shaped cells appeared slightly bent. The additional loss of PBP 4 increased the number of longer cells in the culture. Slow growth caused by a mutation in prfA, a gene found in an operon with the gene encoding PBP 1, was unaffected by the ...Continue Reading

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Citations

Aug 17, 2006·FEMS Microbiology Reviews·Pauline MacheboeufAndréa Dessen
Nov 20, 2016·The FEBS Journal·Alexander J F EganWaldemar Vollmer
Aug 14, 2003·Molecular Microbiology·Kevin D Young
Jan 17, 2017·Veterinary Research Communications·Fatima El AamriFernando Real
Aug 10, 2017·Molecular Microbiology·Jad SassineRichard A Daniel
Feb 5, 2009·Molecular Microbiology·Yoshikazu KawaiJeffery Errington
Mar 28, 2013·Molecular Microbiology·Elitza I TochevaGrant J Jensen
Jan 18, 2019·Nature Communications·Robert M CleverleyRichard J Lewis
Jan 21, 2004·Molecular Microbiology·Dirk-Jan ScheffersJeffery Errington
Feb 4, 2003·Journal of Bacteriology·Derrell C McPherson, David L Popham
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Dirk-Jan Scheffers, Mariana G Pinho
Aug 4, 2009·Journal of Bacteriology·Pradeep VasudevanDavid L Popham
Jul 21, 2004·Journal of Bacteriology·Dirk-Jan Scheffers, Jeffery Errington
Jul 21, 2006·Journal of Bacteriology·Richa PriyadarshiniKevin D Young
Jul 27, 2000·Journal of Bacteriology·J Meador-Parton, D L Popham
Oct 24, 2006·Infection and Immunity·Andrea HamiltonAmanda L Jones
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Jeffery ErringtonDirk-Jan Scheffers
Jul 25, 2019·International Journal of Medical Microbiology : IJMM·Christian MayerHeike Brötz-Oesterhelt
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May 13, 1999·Journal of Bacteriology·L B PedersenP Setlow
Aug 10, 1999·Journal of Bacteriology·P KrishnamurthyS H Phadnis
Apr 29, 1998·Journal of Bacteriology·J Hao, K E Kendrick

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