PMID: 15225136Jul 1, 2004Paper

Phenotypic analysis of c-Kit expression in epithelial monolayers derived from postnatal rat pancreatic islets

The Journal of Endocrinology
Rennian WangN Yashpal

Abstract

The limitation of available islets for transplantation is a major obstacle for the treatment of diabetes through islet therapy. However, islet monolayers expanded ex vivo may provide a source of progenitor cells and a model to help understand islet development from precursor cell types. The existence of progenitor cells within the islets is highly likely, yet, to date, no fully defined or characterized postnatal stem cell has been isolated, expanded or marked. Our study evaluates the expression of progenitor markers, including the haematopoietic stem cell marker c-Kit, in epithelial monolayers derived from postnatal rat islets through immunofluorescence and RT-PCR, and the ability of precursor-rich monolayers to reform islet-like structures. Islets formed confluent monolayers when cultured on a type I collagen gel which lacked endocrine phenotypes but were positive for cytokeratin 20 and contained an increased proportion of proliferating c-Kit-expressing cells, with the proportion reaching a maximum of 45+/-6% at 8 weeks of culture. Evaluation of transcription factors at the mRNA level revealed constant PDX-1, ngn3 and Pax4 expression, while undifferentiated cell markers, such as Oct4 and alpha-fetoprotein, were also detected f...Continue Reading

Citations

Dec 19, 2007·Basic Research in Cardiology·Dong KuangGuoping Wang
Feb 19, 2009·Histochemistry and Cell Biology·Hong WangLiying Li
Aug 2, 2013·International Journal of Biological Sciences·Yan-Ling WuWen Zhang
Nov 20, 2014·Cell Regeneration·Guangming Wu, Hans R Schöler
Jan 15, 2013·International Journal of Endocrinology·Fengxia MaZhongchao Han
Feb 20, 2007·Trends in Molecular Medicine·Shanti T Gangaram-PandayPaul de Vos
Oct 11, 2005·The International Journal of Biochemistry & Cell Biology·Jinming LiRennian Wang
Dec 27, 2007·Cell Stem Cell·Christopher J LengnerRudolf Jaenisch
Jun 10, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yuexiu WuRennian Wang
Oct 1, 2011·The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi·Geom Seog Seo
May 24, 2007·American Journal of Physiology. Endocrinology and Metabolism·Jinming LiRennian Wang
Jan 18, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Zhi-Chao FengRennian Wang
May 10, 2008·Minimally Invasive Therapy & Allied Technologies : MITAT : Official Journal of the Society for Minimally Invasive Therapy·Anna Chiara PiscagliaAntonio Gasbarrini
Dec 12, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Xin LiFang Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Antidiabetic Cellular Plasticity

This feed focuses on mechanisms that underlie cellular plasticity as a treatment for diabetes and other degenerative diseases.

Biomarkers for Diabetes

This feed focuses on the latest research on biomarkers used for monitoring disease progression in diabetes.