PMID: 7524621Jun 1, 1994Paper

Phenotypic and functional analysis of bone marrow progenitor cell compartment in bone marrow failure

British Journal of Haematology
J MaciejewskiN S Young

Abstract

Many laboratory findings have demonstrated that the haemopoietic stem cell compartment is defective in aplastic anaemia (AA). AA bone marrow (BM) and peripheral blood (PB) are profoundly deficient in colony-forming cells, and AA progenitors fail to proliferate in long-term assays even in the presence of an intact stroma. Our study was designed to characterize some quantitative and qualitative aspects of the progenitor cell defect in AA. Using flow cytometric analysis of BM from new AA patients and from those recovering after immunosuppressive therapy, we determined that the numbers of CD34+ and CD33+ cells were markedly decreased in AA. Although PB neutrophil counts did not correlate with BM CD34+ cell numbers in acute disease, there was an association between the overall severity of the disease and the degree of CD34+ cell reduction. A decrease in BM CD33+ cells was a common finding in MDS patients, but reduction in CD34+ cells was found only in some hypoplastic MDS cases. Sorting experiments demonstrated lower plating efficiency for purified CD34+ cells from AA BM in comparison to controls. Thus, diminished colony formation of total BM appeared to result from both quantitative and qualitative defects. Based on the association...Continue Reading

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Citations

Nov 15, 2002·International Journal of Hematology·Edward C Gordon-SmithF M Gibson
Nov 15, 2002·International Journal of Hematology·Jaroslaw P MaciejewskiNeal S Young
Jun 11, 2005·European Journal of Pediatrics·Masaharu AkiyamaYoshikatsu Eto
Oct 26, 2007·Cell and Tissue Research·Stefan Zimmermann, Uwe M Martens
Jan 22, 2004·Archives of Medical Research·Jaroslaw P Maciejewski, Antonio Risitano
Dec 22, 1999·British Journal of Haematology·S RizzoF M Gibson
Aug 10, 2000·British Journal of Haematology·S E Ball
Apr 12, 2001·British Journal of Haematology·J J LeeH J Kim
Dec 12, 2001·British Journal of Haematology·J ScopesF M Gibson
May 8, 1997·The New England Journal of Medicine·N S Young, J Maciejewski
Aug 7, 2007·International Journal of Hematology·Tatsuya Kawaguchi, Hideki Nakakuma
Sep 19, 2003·Wiener klinische Wochenschrift·Klaus Geissler
Jun 5, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Maria M Gramatges, Alison A Bertuch
Mar 31, 2009·Hematology/oncology Clinics of North America·Jeffrey M Lipton, Steven R Ellis
Jul 9, 2003·British Journal of Haematology·Julie FlynnFrances M Gibson
Jan 15, 2008·International Journal of Laboratory Hematology·Y GuC Xu
Feb 1, 2009·Clinical and Translational Science·Irina N ShipounovaNina I Drize
Aug 1, 1995·The Central African Journal of Medicine·P Jacobs
Jul 13, 2004·Experimental Hematology·Siân RizzoFrances M Gibson
Apr 16, 2003·International Journal of Experimental Pathology·Frances M GibsonJohn Turton
Sep 27, 2005·Leukemia Research·Richard D IronsHua Fu
Feb 12, 2005·Pediatric Blood & Cancer·Peter KurreH Joachim Deeg

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