Phenotypic and functional analysis of the HLA-class I-specific inhibitory receptors of natural killer cells isolated from peripheral blood of patients undergoing bone marrow transplantation from matched unrelated donors

The Hematology Journal : the Official Journal of the European Haematology Association
C VitaleL Moretta

Abstract

Limited information is available on the natural killer cell reconstitution after bone marrow transplantation and on the possible role of these cells in graft-versus-host-disease. Blood samples were collected at different time intervals after transplantation. Lymphocytes were analyzed for informative markers by immunocytofluorimetric analysis. Natural killer cells derived from patients undergoing matched unrelated donor transplant were cloned by limiting dilution in the presence of phytohemoagglutinin and IL2. The natural killer cell clones were analyzed for cytolytic activity. In the nine patients analyzed undergoing transplantation from sibling donors, the majority of peripheral blood lymphocytes during the first 80 d after BMT were represented by T lymphocytes, while in the 15 patients undergoing matched unrelated donor transplant natural killer cells consistently outnumbered T lymphocytes. During the early phases after transplantation, most CD56+CD3- natural killer cells did not express CD16 which was expressed at later intervals. Analysis of the inhibitory receptors specific for HLA-class I molecules showed that CD94/NKG2A, specific for HLA-E, unlike normal donors, was expressed by all natural killer cells including the ear...Continue Reading

Citations

Feb 19, 2005·International Journal of Hematology·Junji TanakaMasahiro Imamura
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sally L RogersDixie L Mager
Apr 14, 2010·Journal of Immunotherapy·Annie MichaudMichel Duval
Oct 27, 2016·Current Opinion in Allergy and Clinical Immunology·Emily M Mace
Oct 25, 2008·Seminars in Immunopathology·Jan StorekEddy Roosnek
Sep 13, 2015·Journal of Experimental & Clinical Cancer Research : CR·Alessandra PicardiPatrizio Giacomini
Jun 6, 2003·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·D C Jones, N T Young

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