Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients

Mediators of Inflammation
Suzanne SamaraniAli Ahmad

Abstract

We investigated activation status, cytotoxic potential, and gut homing ability of the peripheral blood Natural Killer (NK) cells in Crohn disease (CD) patients. For this purpose, we compared the expression of different activating and inhibitory receptors (KIR and non-KIR) and integrins on NK cells as well as their recent degranulation history between the patients and age-matched healthy controls. The study was conducted using freshly obtained peripheral blood samples from the study participants. Multiple color flow cytometry was used for these determinations. Our results show that NK cells from treatment-naïve CD patients expressed higher levels of activating KIR as well as other non-KIR activating receptors vis-à-vis healthy controls. They also showed increased frequencies of the cells expressing these receptors. The expression of several KIR and non-KIR inhibitory receptors tended to decrease compared with the cells from healthy donors. NK cells from the patients also expressed increased levels of different gut-homing integrin molecules and showed a history of increased recent degranulation events both constitutively and in response to their in vitro stimulation. Furthermore, treatment of the patients tended to reverse these ...Continue Reading

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Citations

Apr 4, 2021·Cancers·Hansol LeeJames S Wilmott
Jan 19, 2021·Journal of Crohn's & Colitis·Vanessa Zaiatz BittencourtElizabeth J Ryan
Apr 5, 2021·Cellular and Molecular Gastroenterology and Hepatology·Roman KosoyMayte Suárez-Fariñas
Apr 30, 2021·Immunology Letters·Airton Pereira E SilvaGerlinde Agate Platais Brasil Teixeira

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

GraphPad
FlowJo Star
GraphPad Prism

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