Phenotypic and functional characterization of macrophages with therapeutic potential generated from human cirrhotic monocytes in a cohort study

Cytotherapy
Joanna K MooreStuart J Forbes

Abstract

Macrophages have complex roles in the liver. The aim of this study was to compare profiles of human monocyte-derived macrophages between controls and cirrhotic patients, to determine whether chronic inflammation affects precursor number or the phenotype, with the eventual aim to develop a cell therapy for cirrhosis. Infusion of human macrophages in a murine liver fibrosis model demonstrated a decrease in markers of liver injury (alanine transaminase, bilirubin, aspartate transaminase) and fibrosis (transforming growth factor-β, α-smooth muscle actin, phosphatidylserine receptor) and an increase in markers of liver regeneration (matrix metalloproteinases [MMP]-9, MMP-12 and TNF-related weak inducer of apoptosis). CD14+ monocytes were then isolated from controls. Monocytes were matured into macrophages for 7 days using a Good Manufacturing Practice-compatible technique. There was no significant difference between the mean number of CD14+ monocytes isolated from cirrhotic patients (n = 9) and controls (n = 10); 2.8 ± SEM 0.54 × 10(8) and 2.5 ± 0.56 × 10(8), respectively. The mean yield of mature macrophages cultured was also not significantly different between cirrhotic patients and controls (0.9 × 10(8) ± 0.38 × 10(8), with more ...Continue Reading

References

Aug 1, 1973·The British Journal of Surgery·R N PughR Williams
Dec 23, 1999·Current Opinion in Hematology·J L Shepard, L I Zon
Mar 25, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M MalinchocP C ter Borg
Jan 5, 2005·The Journal of Clinical Investigation·Jeremy S DuffieldJohn P Iredale
Jan 30, 2008·Circulation·V BourlierA Bouloumié
Jul 1, 2008·Gastroenterology·Diarmaid Dominic Houlihan, Philip Noel Newsome
Nov 26, 2008·Nature Reviews. Immunology·David M Mosser, Justin P Edwards
Jun 19, 2009·Inflammation & Allergy Drug Targets·Felix HeymannFrank Tacke
Jun 26, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Karlin Raja KarlmarkFrank Tacke
Jan 9, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yury PopovDetlef Schuppan
Jul 14, 2010·Seminars in Nephrology·Jeremy S Duffield
Jul 29, 2010·Seminars in Liver Disease·Thomas A Wynn, Luke Barron
Feb 4, 2011·Journal of the American Society of Nephrology : JASN·Sik LeeLloyd G Cantley
Mar 25, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·James A ThomasStuart J Forbes
Oct 15, 2011·Nature Reviews. Immunology·Peter J Murray, Thomas A Wynn
Oct 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Prakash RamachandranJohn P Iredale
Mar 19, 2013·Journal of Leukocyte Biology·Margaret L Novak, Timothy J Koh
Apr 5, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jean-Philippe PradereRobert F Schwabe
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Thomas G BirdStuart J Forbes
Jan 15, 2014·Journal of Hepatology·Frank Tacke, Henning W Zimmermann
Feb 15, 2014·The American Journal of Pathology·Hanzhou WangPaula K Shireman
Feb 18, 2014·Alimentary Pharmacology & Therapeutics·J K MooreS J Forbes
Feb 26, 2014·Nature Reviews. Immunology·Antonella PellicoroJonathan A Fallowfield
Mar 4, 2014·Advances in Wound Care·Anca Sindrilaru, Karin Scharffetter-Kochanek
Aug 8, 2014·Nature Medicine·Stuart J Forbes, Nadia Rosenthal

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Citations

Aug 16, 2016·Seminars in Cell & Developmental Biology·James W GodwinNadia A Rosenthal
Dec 21, 2017·Inflammation and Regeneration·Atsunori TsuchiyaShuji Terai
Dec 22, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lara CampanaJohn P Iredale
Sep 22, 2018·Cytometry. Part B, Clinical Cytometry·John D M Campbell, Alasdair R Fraser
Aug 16, 2017·World Journal of Gastroenterology : WJG·Veruska Cintia Alexandrino de SouzaSheilla Andrade de Oliveira
May 23, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Martha Lopez-YrigoyenLesley M Forrester
Oct 9, 2019·Nature Medicine·Francesca MoroniStuart J Forbes
Jan 6, 2018·Npj Regenerative Medicine·Sharmin S HaideriLesley M Forrester
Oct 13, 2020·Frontiers in Immunology·Stefaniya Konstantinova BonevaClemens A K Lange
Jun 4, 2021·Nature Reviews. Molecular Cell Biology·Lara CampanaStuart Forbes
Aug 31, 2021·Stem Cells·Somayeh PouyanfardDan S Kaufman

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Methods Mentioned

BETA
biopsy
fluorescence-activated cell sorter
fluorescence
flow cytometry

Clinical Trials Mentioned

NCT02085629

Software Mentioned

Graphpad Prism
GraphPad
SPSS
Adobe Photoshop

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