Phenotypic and functional differentiation of KG-1 into dendritic-like cells

Immunobiology
I TeobaldM J Browning

Abstract

The cell line KG-1 has been used as an in vitro model for human dendritic cell (DC) differentiation. We have investigated the response of KG-1 cells to stimulation with a number of factors known to induce differentiation and/or maturation of DCs in vitro. KG-1 cells showed no differentiation in response to LPS, CpG oligodeoxynucleotide or CD40 ligation. Culture in the presence of TNF-alpha induced some differentiation, but only treatment with PMA and ionomycin (with or without prior culture in GM-CSF and IL-4) induced morphological and phenotypic changes consistent with DC-like maturation, and even these maximally differentiated KG-1 cells showed lower levels of surface marker expression, macromolecular endocytosis, and ability to stimulate in allogeneic MLR compared with in vitro monocyte-derived DCs. Our data show that KG-1 cells differentiate in vitro into cells with DC-like functional characteristics under the influence of strong inducers of cellular activation, but lack the potency of mature DCs in key aspects of professional antigen presenting cells.

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Citations

Jan 12, 2011·Immunology and Cell Biology·Robynn V SchillaceDaniel W Carr
Dec 22, 2011·Journal of Biomedicine & Biotechnology·Jian NingDerwood Pamphilon
Sep 10, 2014·International Reviews of Immunology·Kristian M Hargadon
Sep 19, 2009·Biochemical and Biophysical Research Communications·Amany BalahHeiko Mühl
Nov 29, 2008·Veterinary Microbiology·Isabel Fidalgo-CarvalhoRonald C Montelaro
Mar 20, 2021·Journal for Immunotherapy of Cancer·François AnnaJulien Caumartin
Jun 4, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Reetta VirtakoivuMaija Hollmén

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