Phenotypic and genotypic characterization of Brucella strains isolated from autochthonous livestock reveals the dominance of B. abortus biovar 3a in Nigeria

Veterinary Microbiology
Wilson J BertuR A Ocholi

Abstract

Brucellosis is a worldwide widespread zoonosis caused by bacteria of the genus Brucella. Control of this disease in a given area requires an understanding of the Brucella species circulating in livestock and humans. However, because of the difficulties intrinsic to Brucella isolation and typing, such data are scarce for resource-poor areas. The paucity of bacteriological data and the consequent imperfect epidemiological picture are particularly critical for Sahelian and Sub-Sahara African countries. Here, we report on the characterization of 34 isolates collected between 1976 and 2012 from cattle, sheep and horses in Nigeria. All isolates were identified as Brucella abortus by Bruce-ladder PCR and assigned to biovar 3 by conventional typing. Further analysis by enhanced AMOS-ERY PCR showed that all of them belonged to the 3a sub-biovar, and MLVA analysis grouped them in a cluster clearly distinct from that formed by European B. abortus biovar 3b strains. Nevertheless, MLVA detected heterogeneity within the Nigerian biovar 3a strains. The close genetic profiles of the isolates from cattle, sheep and horses, suggest that, at least in some parts of Nigeria, biovar 3a circulates among animal species that are not the preferential ho...Continue Reading

References

May 1, 1988·Trends in Biochemical Sciences·A Meister
Feb 1, 1969·The Veterinary Record·T N Allsup
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·D G BradleyR T Loftus
Apr 16, 2002·Science·Olivier HanotteJ Edward O Rege
Sep 24, 2004·Veterinary Microbiology·R A OcholiJ O O Bale
Jul 21, 2005·Veterinary Microbiology·Alain A Ocampo-SosaJuan M García-Lobo
Jan 31, 2007·Journal of Microbiological Methods·Sascha Al DahoukHeinrich Neubauer
Jun 11, 2011·Tropical Animal Health and Production·Esther N MuendoHenk L Smits
Aug 13, 2011·Frontiers in Microbiology·Joshua E TurseThomas A Ficht
Mar 19, 2013·Veterinary Microbiology·Moussa SanogoClaude Saegerman
Jan 24, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A S DeanP Pilo
Mar 21, 2015·BioMed Research International·Denis Rwabiita MugiziUlf Magnusson

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Citations

Jan 19, 2016·Frontiers in Cellular and Infection Microbiology·Marcos Mancilla
May 21, 2019·PLoS Neglected Tropical Diseases·Maphuti Betty LedwabaHenriette van Heerden
Jul 18, 2017·Frontiers in Microbiology·Moussa SanogoClaude Saegerman
Apr 4, 2021·Microorganisms·Maphuti Betty LedwabaHenriette van Heerden
Apr 20, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Adrian M Whatmore, Jeffrey T Foster

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