Phenotypic and genotypic comparison of symbiotic and free-living cyanobacteria from a single field site.

Applied and Environmental Microbiology
N J West, David G Adams

Abstract

PCR amplification techniques were used to compare cyanobacterial symbionts from a cyanobacterium-bryophyte symbiosis and free-living cyanobacteria from the same field site. Thirty-one symbiotic cyanobacteria were isolated from the hornwort Phaeoceros sp. at several closely spaced locations, and 40 free-living cyanobacteria were isolated from the immediate vicinity of the same plants. One of the symbiotic isolates was a species of Calothrix, a genus not previously known to form bryophyte symbioses, and the remainder were Nostoc spp. Of the free-living strains, two were Calothrix spp., three were Chlorogloeopsis spp. and the rest were Nostoc spp. All of the symbiotic and all but one of the free-living strains were able to reconstitute the symbiosis with axenic cultures of both Phaeoceros and the liverwort Blasia sp. Axenic cyanobacterial strains were compared by DNA amplification using PCR with either short arbitrary primers or primers specific for the regions flanking the 16S-23S rRNA internal transcribed spacer. With one exception, the two techniques produced complementary results and confirmed for the first time that a diversity of symbiotic cyanobacteria infect Phaeoceros in the field. Symbionts from adjacent colonies were di...Continue Reading

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Citations

Nov 9, 2019·Microbiology Resource Announcements·Marie-Josée H HalsørBjørn Altermark
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·John C Meeks, Jeff Elhai
Nov 19, 2004·Archives of Microbiology·Mette M SvenningUlla Rasmussen
Feb 10, 2009·Microbiology·Elisabete ValérioRogério Tenreiro
Aug 30, 2001·Applied and Environmental Microbiology·J L CostaP Lindblad
May 1, 2006·American Journal of Botany·Juan Carlos Villarreal A, Karen Sue Renzaglia
Feb 17, 2010·Nature Reviews. Microbiology·Monika Bright, Silvia Bulgheresi
May 11, 2005·Microbial Ecology·C A Crispim, C C Gaylarde
Mar 6, 2008·International Journal of Systematic and Evolutionary Microbiology·Dimitra PapaefthimiouStefano Ventura
May 18, 2010·Journal of Basic Microbiology·Nooruddin ThajuddinMuthukumaran Gunasekaran
Mar 2, 2016·Environmental Microbiology Reports·Carolina Cornejo, Christoph Scheidegger
Dec 21, 2016·SpringerPlus·Harshini M LiyanageNaduviladath V Chandrasekaran
Mar 20, 2018·Molecular Biology and Evolution·Denis WarshanUlla Rasmussen
Oct 1, 2003·The New Phytologist·Nora WirtzBurkhard Schroeter

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