Phenotypic and genotypic identification of enterococci isolated from canals of root-filled teeth with periapical lesions

Oral Microbiology and Immunology
E T PinheiroD B Drucker

Abstract

The objectives of the present study were to identify enterococcal species isolated from the canals of root-filled teeth with periapical lesions using biochemical and molecular techniques, and to investigate the genetic diversity of the isolates. Twenty-two Enterococcus strains, isolated from the canals of root-filled teeth with persisting periapical lesions, were identified to species level using rapid ID 32 STREP galleries and partial 16S rDNA sequencing. To subtype the strains, genomic DNA from the isolates was analyzed by pulsed field gel electrophoresis (PFGE) after digestion with SmaI. Intragenic regions of two genes, ace and salA, were sequenced for further differentiation of the isolates. All strains were identified as Enterococcus faecalis by both commercial kit and partial 16S rDNA sequencing. PFGE with SmaI of 22 isolates demonstrated 18 macrorestriction profiles, whereas 13 distinct genotypes were identified after analysis of the ace and salA composite sequences. Most of the isolates from distinct patients had different PFGE profiles. Moreover, in two cases, different E. faecalis strains were found in different root-filled teeth from the same mouth. E. faecalis was the only enterococcal species isolated from the cana...Continue Reading

References

Aug 1, 1992·Oral Microbiology and Immunology·T E RamsJ Slots
Jan 1, 1990·Clinical Microbiology Reviews·B E Murray
Aug 1, 1987·Journal of Dental Research·M Haapasalo, D Orstavik
Apr 1, 1988·Oral Surgery, Oral Medicine, and Oral Pathology·Y B Wahlin, A K Holm
Dec 1, 1993·Oral Microbiology and Immunology·G DahlénA J Möller
Aug 1, 1996·Computer Applications in the Biosciences : CABIOS·R D Page
Feb 25, 1998·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·G SundqvistU Sjögren
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·M C MaidenB G Spratt
May 15, 1999·Journal of Clinical Microbiology·D M Olive, P Bean
Oct 12, 2000·Clinical Microbiology Reviews·L M MundyM Gilmore
Nov 4, 2000·Journal of Clinical Microbiology·D TurabelidzeA Sulakvelidze
Jan 12, 2001·Oral Microbiology and Immunology·G DahlénC Reit
Feb 7, 2001·Journal of Clinical Microbiology·S AngelettiG Dicuonzo
May 11, 2001·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·H H HancockJ Moiseiwitsch
Aug 3, 2001·International Endodontic Journal·R M Love
May 3, 2002·International Endodontic Journal·M EvansD Figdor
Mar 27, 2003·International Endodontic Journal·E T PinheiroF J Souza-Filho
Jun 26, 2003·Oral Microbiology and Immunology·D FigdorG Sundqvist
Oct 15, 2003·Trends in Microbiology·Rachel Urwin, Martin C J Maiden
Jan 13, 2004·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·José F Siqueira, Isabela N Rôças
Feb 12, 2004·Oral Microbiology and Immunology·B P F A GomesF J Souza-Filho
Apr 27, 2004·Journal of Endodontics·Isabela N RôçasKátia R N Santos
Oct 8, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Güven Kayaoglu, Dag Ørstavik
Dec 23, 2004·Oral Microbiology and Immunology·C M SedgleyG Dahlén

❮ Previous
Next ❯

Citations

Oct 12, 2007·Journal of Oral Science·Ali MahmoudpourNaser Asl-Aminabadi
Mar 14, 2020·Journal of Global Infectious Diseases·Sonia Bhonchal BhardwajJyoti Sharma

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.