Phenotypic and lipidomic characterization of primary human epidermal keratinocytes exposed to simulated solar UV radiation

Journal of Dermatological Science
Núria DalmauCarmen Bedia

Abstract

Ultraviolet (UV) radiation is known to be one of the most important environmental hazards acting on the skin. The most part of UV radiation is absorbed in the epidermis, where keratinocytes are the most abundant and exposed cell type. Lipids have an important role in skin biology, not only for their important contribution to the maintenance of the permeability barrier but also for the production and storage of energy, membrane organization and cell signalling functions. However, the effects on the lipid composition of keratinocytes under UV radiation are little explored. The present work aims to explore the effects on the phenotype and lipid content of primary human keratinocytes exposed to simulated solar UV radiation. Keratinocytes were exposed to a single (acute exposure) and repeated simulated solar UV irradiations for 4 weeks (chronic exposure). Cell viability and morphology were explored, as well as the production of reactive oxygen species. Then, lipid extracts were analysed through liquid chromatography coupled to mass spectrometry (LC-MS) and the data generated was processed using the ROIMCR chemometric methodology together with partial least squares discriminant analysis (PLS-DA), to finally reveal the most relevant l...Continue Reading

Citations

May 19, 2019·BMC Bioinformatics·Eva GorrochateguiRomà Tauler
Sep 13, 2020·International Journal of Molecular Sciences·Wojciech ŁuczajElżbieta Skrzydlewska
Dec 19, 2020·Clinical, Cosmetic and Investigational Dermatology·Sudhir M BaswanNathan Stern
May 29, 2021·Scientific Reports·Xiaoyu YangLei Liu

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