Phenotypic and molecular analysis of the effect of 20-hydroxyecdysone on the human filarial parasite Brugia malayi

International Journal for Parasitology
Amruta S MhashilkarThomas R Unnasch

Abstract

A homologue of the ecdysone receptor has been identified and shown to be responsive to 20-hydroxyecdysone in Brugia malayi. However, the role of this master regulator of insect development has not been delineated in filarial nematodes. Gravid adult female B. malayi cultured in the presence of 20-hydroxyecdysone produced significantly more microfilariae and abortive immature progeny than control worms, implicating the ecdysone receptor in regulation of embryogenesis and microfilarial development. Transcriptome analyses identified 30 genes whose expression was significantly up-regulated in 20-hydroxyecdysone-treated parasites compared with untreated controls. Of these, 18% were identified to be regulating transcription. A comparative proteomic analysis revealed 932 proteins to be present in greater amounts in extracts of 20-hydroxyecdysone-treated adult females than in extracts prepared from worms cultured in the absence of the hormone. Of the proteins exhibiting a greater than two-fold difference in the 20-hydroxyecdysone-treated versus untreated parasite extracts, 16% were involved in transcriptional regulation. RNA interference (RNAi) phenotype analysis of Caenorhabditis elegans orthologs revealed that phenotypes involved in d...Continue Reading

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Citations

Apr 21, 2017·PLoS Neglected Tropical Diseases·Sara LustigmanElodie Ghedin
Jun 15, 2016·PLoS Neglected Tropical Diseases·Amruta S MhashilkarThomas R Unnasch
Jun 24, 2020·PLoS Neglected Tropical Diseases·Alexandra GroteElodie Ghedin
Sep 1, 2020·PLoS Neglected Tropical Diseases·Canhui LiuThomas R Unnasch
Oct 17, 2017·Trends in Parasitology·Sasisekhar BennuruThomas B Nutman

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