Phenotypic and molecular characterization of clinical isolates of Acinetobacter baumannii isolated from Delhi, India

Annals of Clinical Microbiology and Antimicrobials
Dabet RyngaMonorama Deb

Abstract

Acinetobacter has gained importance as a multi-drug resistant and hence a difficult to treat pathogen. This study was done to characterize our isolates with respect to drug resistance and presence of beta-lactamases which is a major mechanism of resistance and to type using RAPD and MLST so that comparison of our clones can be made with the existing international clones. 100 isolates recovered from clinical samples from two hospitals in Delhi were tested for their susceptibility against major groups of antimicrobials. The resistant isolates were screened and confirmed phenotypically for presence of ESBL, MBL and AmpC and MBLs also by PCR. The isolates were typed by RAPD and MLST. Out of the 100 isolates, 91, 78 and 2 % were MDR, XDR and PDR respectively. 97, 100 and 85 were screen positive for ESBL, AmpC and MBL respectively. Of these, 38.1 % were confirmed phenotypically to produce ESBL, 99 % produced AmpC and 29.4 % produced MBL comprising of GIM, VIM, SIM and IMP. MLST showed known STs 110, 188, 146, 69, 103, 108 and 194. Eight new STs were encountered. The RAPD showed a high degree of genetic variability among the isolates. Majority of our isolates were MDR, producing one or more types of beta-lactamases. We encountered dru...Continue Reading

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Citations

Aug 8, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Afaf Mohamed Samy El BannahSherifa Tarek Bassiouni Salem
Nov 1, 2016·Clinical Microbiology Reviews·Li-Yang HsuPaul Anantharajah Tambyah
Mar 23, 2017·Journal of Medical Microbiology·Tarek A A MoussaSybren de Hoog
Mar 19, 2020·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·M C UgwuC O Esimone
Nov 12, 2020·Revista Da Sociedade Brasileira De Medicina Tropical·Mariana Neri Lucas KuriharaSimone Simionatto
Dec 9, 2021·Expert Review of Anti-infective Therapy·Prashant ChandraUnnikrishnan Mk

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

MLST
Gelcompar
Bio Edit Sequence alignment editor
Amplified restriction analysis ( ARDRA )
eBURST

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