Phenotypic and Transcriptomic Response of the Grasshopper Oedaleus asiaticus (Orthoptera: Acrididae) to Toxic Rutin

Frontiers in Physiology
Xunbing HuangBabar Hussain Chang

Abstract

Rutin, a widely distributed phytochemical flavonoid, can be used to control insect pests. In this study, we studied the growth performance of the grasshopper Oedaleus asiaticus Bey-Bienko given xenobiotic rutin using feeding experiments and transcriptomic analysis. O. asiaticus had reduced body size, lower survival rate, and reduced growth performance when fed with xenobiotic rutin. Rutin-fed nymphs had large variation in gene expression profiles, with a total of 308 genes significantly upregulated and 287 genes downregulated. The upregulated genes were significantly enriched in stress resistance-, immune-, and detoxification-related biological processes and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Downregulated genes mainly involved cuticle biosynthesis and nutrition metabolism-related pathways. The quantitative real-time PCR (qRT-PCR) analysis of 15 candidate genes also produced results consistent with the transcriptome data. These results suggested that grasshoppers' capacity for biosynthesis and nutrition metabolism decreased, and stress resistance and metabolized capacity to toxic substances were significantly induced when O. asiaticus was fed on xenobiotic rutin. Rutin, as a phytotoxin, had detrimental...Continue Reading

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Citations

Dec 30, 2020·Current Opinion in Insect Science·Marilou VandenholeThomas Van Leeuwen
Nov 10, 2021·Archives of Insect Biochemistry and Physiology·ShangHong Xin, WenJun Zhang

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Datasets Mentioned

BETA
SRP072969

Methods Mentioned

BETA
RNA-seq
RNA Assay
PCR
environmental stress

Software Mentioned

Rsubread
Blast2GO
DESeq2 R
Trinity
Omics
Primer
BLASTX
SOAPaligner
KOBAS
GOseq R

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