Phenotypic characterization of Ewing sarcoma cell lines with monoclonal antibodies

Journal of Cellular Biochemistry
M LipinskiT Tursz

Abstract

The histogenesis of Ewing sarcoma, the second most frequent bone tumor in humans, remains controversial. Four Ewing cell lines were analyzed by immunological methods. A panel of antibodies directed to T, B, and myelomonocytic markers gave negative results. Surface antigens recognized on Ewing cells were found to be related to the neuroectoderm lineage. Ganglioside GD2, a marker of neuroectodermal tissues and tumors, was present on all lines. These were also stained by the mouse monoclonal antibody HNK-1, which detects a carbohydrate epitope present on several glycoconjugates of the nervous system, including two glycoproteins, the myelin-associated glycoprotein and the neural cell-adhesion molecule (N-CAM), and an acidic glycolipid of the peripheral nervous system. The P61 monoclonal antibody, which reacts with a peptide moiety of N-CAM, and a rabbit antiserum, raised to purified mouse N-CAM and not recognizing the HNK-1-defined epitope, were also reactive. By contrast, all antibodies specific for hematopoietic cell surface antigens were totally negative. Besides these antigenic features, Ewing sarcoma cells are characterized by a specific t(11;22)(q24;q12) translocation also observed in neuroepithelioma, a neuroectodermal tumor...Continue Reading

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Citations

Jun 15, 2010·Oncogene·E C ToomeyS L Lessnick
Jan 1, 1991·Annals of the New York Academy of Sciences·K L Crossin
Aug 5, 2008·Diagnostic Pathology·Rosa NogueraAntonio Llombart-Bosch
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Jan 19, 2021·International Journal of Cancer. Journal International Du Cancer·Wilhelm Gerhard DirksJürgen Becker

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