Phenotypic characterization of Gardnerella vaginalis subgroups suggests differences in their virulence potential

PloS One
Migle JanulaitieneMilda Pleckaityte

Abstract

The well-known genotypic and phenotypic diversity of G. vaginalis resulted in its classification into at least four subgroups (clades) with diverse genomic properties. To evaluate the virulence potential of G. vaginalis subgroups, we analyzed the virulence-related phenotypic characteristics of 14 isolates of clade 1, 12 isolates of clade 2, 8 isolates of clade 4 assessing their in vitro ability to grow as a biofilm, produce the toxin vaginolysin, and express sialidase activity. Significant differences in VLY production were found (p = 0.023), but further analysis of clade pairs did not confirm this finding. The amount of biofim did not differ significantly among the clades. Analysis of sialidase activity indicated statistically significant differences among the clades (p < 0.001). Production of active recombinant G. vaginalis sialidase demonstrated the link between the sld gene and enzymatic activity, which may be differentially regulated at the transcriptional level. Statistical classification analysis (random forests algorithm) showed that G. vaginalis clades could be best defined by the profiles of two phenotypic characteristics: sialidase activity and vaginolysin production. The results of principal component analysis and h...Continue Reading

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Citations

Dec 4, 2019·Current Opinion in Infectious Diseases·Christina A MuznyMelissa M Herbst-Kralovetz
Jul 23, 2020·Infectious Diseases in Obstetrics and Gynecology·Kayla PillayNathlee Abbai
May 31, 2019·Journal of the International AIDS Society·Salim S Abdool KarimBrent L Williams
Nov 19, 2019·Frontiers in Cellular and Infection Microbiology·Salahuddin KhanJanet E Hill
May 12, 2020·Frontiers in Cellular and Infection Microbiology·Sydney MorrillAmanda L Lewis
Sep 2, 2020·Pathogens·Aistė BulavaitėMilda Pleckaityte
Apr 8, 2020·Frontiers in Cellular and Infection Microbiology·Hyunsul JungMarleen M Kock
Jul 17, 2020·Frontiers in Cellular and Infection Microbiology·Suzanna Carter FrancisAnne Buvé
Jan 31, 2020·Frontiers in Cellular and Infection Microbiology·Milda Pleckaityte
Aug 28, 2019·Trends in Microbiology·Marina PekmezovicBernhard Hube
Jan 28, 2021·Pathogens·Erin M GarciaKimberly K Jefferson
Mar 4, 2021·Microbial Genomics·Brett A TortelliJustin C Fay
Apr 30, 2021·Fungal Biology·Radoslav AbrashevMaria Angelova
Jun 3, 2021·Pharmaceutics·Louise CarsonRobert Karl Malcolm
Dec 7, 2021·Frontiers in Cellular and Infection Microbiology·Lúcia G V SousaNuno Cerca

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Methods Mentioned

BETA
biotyping
genotyping
PCR
electrophoresis
PCA
gel after filtration
ELISA

Software Mentioned

R
R package “ ranger ”
R package “ caret ”

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