Phenotypic comparison of multiple monocyte-related populations in murine peripheral blood and bone marrow

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Annette J Schlueter, Judith K Glasgow

Abstract

Monocyte subsets are not well defined in murine peripheral blood (PB). Monocyte-related populations could also be located in bone marrow (BM), but studies correlating monocyte populations found in these two tissues are lacking. This study simultaneously analyzed PB and BM to phenotypically define multiple monocyte-related subsets in each location. Murine PB and BM cells were simultaneously stained for monocyte-related populations, using five-color flow cytometry. Relevant subsets were defined on the basis of Ly-6C, CD11b, and wheat germ agglutinin phenotype in addition to light-scatter characteristics. These populations were extensively characterized for the expression of other myeloid and dendritic cell markers, adhesion molecules, chemokine receptors, and growth factor receptors. Six monocyte-related populations were defined, three each in BM and PB. No identical populations were found between the two tissues. Two BM populations and one PB population have heterogeneous expression of many markers, suggesting additional complexity among monocyte-related subsets. The murine monocytic series comprises multiple subsets, differing between PB and BM. This study defines and extensively phenotypes six of these populations, providing p...Continue Reading

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Citations

Feb 28, 2008·The Journal of Trauma·Guillermo M AlbaicetaFrancisco Taboada
Sep 11, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·David M Duriancik, Kathleen A Hoag
Apr 28, 2010·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A E GelmanD Kreisel
Mar 15, 2011·Current Protocols in Immunology·Violeta ChituE Richard Stanley

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