PMID: 11327081May 1, 2001Paper

Phenotypic comparison of periodontal ligament cells in vivo and in vitro

Journal of Periodontal Research
P LekicC A McCulloch

Abstract

The mammalian periodontal ligament contains heterogeneous populations of connective tissue cells, the precise function of which is poorly understood. Despite close proximity to bone and the application of high amplitude physical forces, cells in the periodontal ligament (PL) are capable of expressing regulatory factors that maintain PL width during adult life. The study of PL homeostasis and PL cell differentiation requires culture and phenotypic methods for precise characterization of PL cell populations, in particular those cells with an inherently osteogenic program. Currently it is unknown if cells cultured from the PL are phenotypically similar to the parental cells that are present in the tissues. We have compared the phenotype of cells in vivo with cells derived from the PL and expanded in vitro to assess the general validity of in vitro models for the study of phenotypic regulation in vivo. Rat PL cells were isolated by either scraping the root of the extracted first mandibular molars (Group A), or by scraping the alveolar socket following extraction of first mandibular molars (Group B), or by obtaining a mixture of cells after disaggregating a block of tissue consisting of first mandibular molar, PL and the surrounding...Continue Reading

Citations

Jul 13, 2002·Journal of Biomedical Materials Research·Tarek Y El SayeghChristopher A G McCulloch
Apr 20, 2011·Journal of Orofacial Orthopedics = Fortschritte Der Kieferorthopädie : Organ/official Journal Deutsche Gesellschaft Für Kieferorthopädie·Sandra HornikelHeiner Wehrbein
Oct 13, 2006·Calcified Tissue International·S GronthosP M Bartold
Feb 14, 2012·Histochemistry and Cell Biology·Yoshiaki NomuraNobuhiro Hanada
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Oct 6, 2012·Dental Materials Journal·Kanitsak BoonanantanasarnWanida Sripairojthikoon
Apr 23, 2013·Clinical Oral Investigations·Nuersailike AbuduwaliAndreas Jäger
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Aug 14, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jin LiuJianzhong Gou
Dec 14, 2011·Journal of Endodontics·Young-Seok ParkWon-Jun Shon
Aug 30, 2008·Matrix Biology : Journal of the International Society for Matrix Biology·Takayuki HayamiSunil Kapila
Nov 1, 2008·Journal of Periodontal Research·T ItayaM Ueda
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May 21, 2009·Journal of Periodontal Research·S M San MiguelI Kalajzic

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