Phenotypic Correlates of HIV-1 Macrophage Tropism

Journal of Virology
Kathryn Twigg ArrildtRonald Swanstrom

Abstract

HIV-1 is typically CCR5 using (R5) and T cell tropic (T-tropic), targeting memory CD4(+) T cells throughout acute and chronic infections. However, viruses can expand into alternative cells types. Macrophage-tropic (M-tropic) HIV-1 variants have evolved to infect macrophages, which have only low levels of surface CD4. Most M-tropic variants have been isolated from the central nervous system during late-stage chronic infection. We used the HIV-1 env genes of well-defined, subject-matched M-tropic and T-tropic viruses to characterize the phenotypic features of the M-tropic Env protein. We found that, compared to T-tropic viruses, M-tropic viruses infect monocyte-derived macrophages (MDMs) on average 28-fold more efficiently, use low-density CD4 more efficiently, have increased sensitivity to soluble CD4 (sCD4), and show trends toward sensitivity to some CD4 binding site antibodies but no difference in sensitivity to antibodies targeting the CD4-bound conformation. M-tropic viruses also displayed a trend toward resistance to neutralization by monoclonal antibodies targeting the V1/V2 region of Env, suggesting subtle changes in Env protein conformation. The paired M- and T-tropic viruses did not differ in autologous serum neutraliza...Continue Reading

References

Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D R BurtonR A Lerner
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E S DaarD D Ho
Oct 1, 1986·Journal of the Neurological Sciences·G Keir, E J Thompson
Jul 1, 1996·AIDS Research and Human Retroviruses·S R LewinS M Crowe
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·C C BleulC R Mackay
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·B LeeR W Doms
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·T L HoffmanR W Doms
May 3, 2002·Nature·Daniel C DouekRichard A Koup
Mar 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Douglas D RichmanChristos J Petropoulos
Mar 21, 2003·Nature·Xiping WeiGeorge M Shaw
Mar 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zhihai SiJoseph G Sodroski
Apr 20, 2004·AIDS·Marlén M I Aasa-ChapmanAine McKnight
Aug 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yoshiaki NishimuraMalcolm A Martin
Dec 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Simon D W FrostDouglas D Richman

❮ Previous
Next ❯

Citations

Mar 11, 2016·Cell Host & Microbe·Quentin J Sattentau, Mario Stevenson
Jul 23, 2016·Current Opinion in Virology·Nicolas HuotMichaela Müller-Trutwin
Feb 2, 2018·Journal of Leukocyte Biology·Sarah B Joseph, Ronald Swanstrom
Jan 31, 2018·Retrovirology·Gert van ZylMary F Kearney
Dec 19, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sarah B JosephRonald Swanstrom
Jun 11, 2019·PLoS Biology·Cody J WarrenSara L Sawyer
Jan 10, 2020·AIDS Research and Human Retroviruses·Olubusuyi Moses AdewumiRonald Swanstrom
Jun 26, 2020·Frontiers in Microbiology·Furkan GuvencScott D Gray-Owen
Jun 17, 2016·Journal of Virology·Sarah R DiNapoliJason M Brenchley
Dec 23, 2017·Current HIV Research·Olivia D Council, Sarah B Joseph
Dec 7, 2018·PLoS Pathogens·Philippe ColinBernard Lagane
Jan 11, 2020·Frontiers in Microbiology·Shane D FalcinelliNancie M Archin
Oct 20, 2020·Frontiers in Immunology·Alexander OschwaldDaniel Erny

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.