Phenotypic expression of marrow cells when grown on various substrata

Journal of Cellular Biochemistry
A FriedD Benayahu

Abstract

Our aim was to study the role of various extracellular matrices (ECM) on growth and differentiation of marrow stromal cells in vitro. Morphology changes, gene expression, and enzymatic activities were monitored in stromal osteoblastic MBA-15 and adipocytic 14F1.1 cells. These stromal cells were plated on dishes precoated with different substrata, such as matrigel (basement membrane), collagen type I, and endothelial ECM, and compared with cells plated on protein-free dishes. Striking morphological differences were observed when the cells grew on these different substrata. Changes in cell shape and growth also led to differential mRNA expression and enzymatic activities. When MBA-15 cells were plated on collagen, there was a decrease in mRNA for alkaline phosphatase (ALK-P), osteopontin (OP), and osteonectin (ON), and an increase in mRNA for procollagen (I). A differential effect was noted on 14F1.1 cells, the mRNA for ALK-P increased, the expressions of OP and ON lowered, and no expression for procollagen (I) was monitored. MBA-15 cells cultured on matrigel had decreased mRNA for ALK-P and OP, while they had increased ON mRNA expression and remained unchanged for procollagen I. No change in mRNA expression by 14F1.1 cells was m...Continue Reading

References

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Citations

Jun 2, 2005·Journal of Materials Science. Materials in Medicine·Shyh Ming KuoShwu Jen Chang
Aug 19, 2007·Journal of Materials Science. Materials in Medicine·Shyh Ming KuoShwu Jen Chang
May 22, 2008·Tissue Engineering. Part a·Cristina Martínez-RamosJosé Miguel Soria
May 10, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K D HankensonP Bornstein
Mar 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kurt D Hankenson, Paul Bornstein

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