Phenotypic features of BHK-21 cells used for production of foot-and-mouth disease vaccine

Biologicals : Journal of the International Association of Biological Standardization
M AmadoriC Berneri

Abstract

BHK-21 c13 monolayer and suspension cells were investigated with regard to some phenotypic features which could bear on the quality of foot-and-mouth disease virus (FMDV) antigen produced in them. Despite good viability, suspension cells differed from monolayer cells in fundamental features of susceptibility to FMDV. Most important, FMD virus particles grown in suspension cells at high passage levels were shown to be largely degraded following inactivation with an aziridine compound. Suspension cells were characterised by a downregulation in the surface expression of both alpha 5 and alpha V integrin chains. According to the results of binding assays, both integrins could act as FMDV receptors on BHK-21 c13 cells. Reduced surface expression of integrins was correlated with disappearance of actin stress fibres, which would play a role in regular encapsidation of viral RNA and hence in stability of virus particles. With regard to FMD vaccine production, practical suggestions are put forward to evaluate the quality of BHK-21 c13 cells and FMDV Ag, which must prove stable during downstream processing.

Citations

Dec 3, 2014·Biologicals : Journal of the International Association of Biological Standardization·Jitendra K BiswalBramhadev Pattnaik
Jul 18, 2014·Journal of Virology·Lisbeth Ramírez-CarvajalTeresa de los Santos
Sep 29, 2020·Frontiers in Immunology·Inmaculada Sanclemente-AlamanUlises Gómez-Pinedo

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