Phenotypic Modulation of Cultured Primary Human Aortic Vascular Smooth Muscle Cells by Uremic Serum

Frontiers in Physiology
Violeta Cazaña-PérezDiego Alvarez de la Rosa

Abstract

Patients with chronic kidney disease (CKD) have a markedly increased incidence of cardiovascular disease (CVD). The high concentration of circulating uremic toxins and alterations in mineral metabolism and hormone levels produce vascular wall remodeling and significant vascular damage. Medial calcification is an early vascular event in CKD patients and is associated to apoptosis or necrosis and trans-differentiation of vascular smooth muscle cells (VSMC) to an osteogenic phenotype. VSMC obtained from bovine or rat aorta and cultured in the presence of increased inorganic phosphate (Pi) have been extensively used to study these processes. In this study we used human aortic VSMC primary cultures to compare the effects of increased Pi to treatment with serum obtained from uremic patients. Uremic serum induced calcification, trans-differentiation and phenotypic remodeling even with normal Pi levels. In spite of similar calcification kinetics, there were fundamental differences in osteochondrogenic marker expression and alkaline phosphatase induction between Pi and uremic serum-treated cells. Moreover, high Pi induced a dramatic decrease in cell viability, while uremic serum preserved it. In summary, our data suggests that primary c...Continue Reading

References

May 1, 1979·The American Journal of Medicine·L S IbelsR Weil
Apr 1, 1993·The Journal of Clinical Investigation·K BoströmL L Demer
Mar 21, 1998·Transplant Immunology·S M Schwartz
Feb 5, 1999·Circulation Research·T WadaC M Giachelli
Jan 29, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·U SchwarzK Amann
Sep 29, 2000·Circulation Research·S JonoC M Giachelli
Aug 15, 2002·International Journal of Epidemiology·Linda L Demer
Mar 5, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Kerry L TysonCatherine M Shanahan
Nov 25, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Tatjana CoricCecilia M Canessa
May 22, 2004·Journal of the American Society of Nephrology : JASN·Koba A LomashviliW Charles O'Neill
May 25, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Moeen AbedinLinda L Demer
Sep 18, 2004·Circulation Research·Sharon M Moe, Neal X Chen
Dec 8, 2004·Journal of the American Society of Nephrology : JASN·Cecilia M Giachelli
Dec 31, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Masahide MizobuchiTadao Akizawa
Sep 29, 2006·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Neal X ChenSharon M Moe
Nov 4, 2006·Annual Review of Medicine·Marcello Tonelli, Marc A Pfeffer
Feb 27, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Ricardo Villa-BellostaVíctor Sorribas
Sep 28, 2007·The New England Journal of Medicine·Timothy W Meyer, Thomas H Hostetter
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Feb 14, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Ricardo Villa-Bellosta, Víctor Sorribas
Jun 5, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Ellen NevenPatrick C D'Haese
Jun 18, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Rafael KramannRebekka K Schneider
Aug 16, 2011·Lancet·Andrew S Levey, Josef Coresh
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Feb 8, 2013·Kidney International·Julia J SciallaUNKNOWN Chronic Renal Insufficiency Cohort Study Investigators
Aug 24, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Matthew H CrouthamelCecilia M Giachelli
Aug 20, 2014·TheScientificWorldJournal·Kuo-Cheng LuWen-Chih Liu
Sep 12, 2014·Pflügers Archiv : European journal of physiology·Pilar CidadJosé Ramón López-López
Jan 2, 2015·Frontiers in Bioscience (Landmark Edition)·M Alexandra MonroyEric T Choi
Jun 30, 2015·Journal of Atherosclerosis and Thrombosis·Tomohiro SonouTakashi Shigematsu
Dec 18, 2015·Methods in Molecular Biology·Edward R Smith
May 14, 2016·Methods in Molecular Biology·Jessal J PatelRichard C M Siow
Oct 30, 2016·Seminars in Cell & Developmental Biology·Chia-Feng LiuVéronique Lefebvre
Nov 17, 2016·Bone·Shunsuke Yamada, Cecilia M Giachelli
Feb 9, 2017·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Kerstin BenzKerstin Amann

❮ Previous
Next ❯

Citations

May 31, 2018·Toxins·Lucie HénautZiad A Massy
Oct 9, 2019·Expert Review of Cardiovascular Therapy·Takamasa IwaiSatoshi Yasuda
Nov 6, 2020·Journal of Cellular and Molecular Medicine·Jing-Wei GaoPin-Ming Liu
Nov 12, 2020·Cells·Jana HolmarSetareh Orth-Alampour
Jul 11, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Isabel Amador-MartínezJonatan Barrera-Chimal

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
PCR

Software Mentioned

MatLab
Imaging Workbench
ImageJ

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis