Phenotypic modulation of hamster acinar cells by culture in collagen matrix

Experimental Cell Research
S YuanL Rosenberg

Abstract

The aim of this study was to assess the effect of different culture conditions on the survival and morphological phenotype of cultured acinar cells. Acinar fragments isolated from hamster pancreas were embedded in rat-tail collagen. Four groups were established: Medium 1-5% NuSerum + basic medium (basic medium = DMEM/F12 supplemented with dexamethasone, 3-isobutyl-2-methylxanthine, and antibiotics); Medium 2-10% NuSerum + basic medium. Medium 3-Medium 2 supplemented with epidermal growth factor and cholera toxin; and Medium 4:-Medium 3 supplemented with soybean trypsin inhibitor. Freshly isolated acinar cells were retrieved morphologically intact. In Medium 1, more than 80% of cells retained a normal histological appearance at 34 days in culture. Immunostaining for amylase was observed at the apical pole of the cells. The remaining cells showed variable degrees of degeneration. In Medium 2, approximately 50% of acinar cells appeared normal at 34 days in culture, while the remainder were severely degenerated. A few cystic structures were also observed. Positive immunostaining for amylase was limited to the cells with a normal histological appearance. The cells grown in Media 3 and 4 had similar courses of morphological changes. ...Continue Reading

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Citations

Mar 16, 2004·Pediatric Diabetes·Rohan K HumphreyAlberto Hayek
Dec 26, 2015·Developmental Cell·Nilotpal Roy, Matthias Hebrok
Oct 17, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Matthew M RiopelRennian Wang
Jan 11, 2005·World Journal of Gastroenterology : WJG·Yun-Fu CuiMichael D Menger
Jul 18, 2002·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·A L Means, S D Leach
Mar 11, 2004·The International Journal of Biochemistry & Cell Biology·Cale N StreetGregory S Korbutt
Dec 7, 2007·Gastroenterology·Stephen F Konieczny, Steven D Leach

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