Phenotypic, molecular, and virulence characterization of entomopathogenic fungi, Beauveria bassiana (Balsam) Vuillemin, and Metarhizium anisopliae (Metschn.) Sorokin from soil samples of Ethiopia for the development of mycoinsecticide.

Heliyon
Amha GebremariamFassil Assefa

Abstract

Fungal entomopathogens are the most effective biocontrol agents against insect pests in the natural ecosystem. This study was conducted for phenotypic, molecular, and virulence characterization of locally isolated entomopathogenic fungi from soil samples of six localities in Ethiopia. Entomopathogenic fungi were isolated from 120 soil samples with the galleria baiting method. A total of 65 (54.2%) entomopathogenic fungal isolates belongs to Beauveria spp and Metarhizium spp were identified based on cultural and morphological features. All isolates were pre-screened based on germination, vegetative growth rate, and spore production as fungal in-vitro virulence determinates. Isolate AAUKB-11 displayed the peak germination of 99.67% and isolate AAUMFB-77 achieved the highest radial growth rate of 3.43 mm day-1 with the highest sporulation 4.60 × 108spores/ml. The phylogenetic analysis of ITS-rDNA confirmed that 7 isolates were identified as B. bassiana and 5 isolates were categorized into M. anisopliae. Selected B. bassiana and M. anisopliae strains were evaluated for their pathogenicity efficiency against G. mellonella larvae and caused 86.67%-100% mortality. The mortality rates of G. mellonella larvae peaked at 100% with 4(33.33...Continue Reading

References

May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Sep 12, 2012·World Journal of Microbiology & Biotechnology·Thet Thet MarSaisamorn Lumyong
Dec 3, 2015·Journal of Invertebrate Pathology·Claudio A Valero-JiménezJan A L van Kan
Jul 28, 2016·Journal of Invertebrate Pathology·Abdessamad ImoulanYi-Jian Yao
Apr 24, 2019·Proceedings of the National Academy of Sciences of the United States of America·Eric H CliftonAnn E Hajek

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