Phenotypic screening and molecular characterization of carbapenemase-producing Gram-negative bacilli recovered from febrile neutropenic pediatric cancer patients in Egypt

PloS One
Noha A KamelKhaled Mohamed Aboshanab

Abstract

Infections with carbapenem-resistant Gram-negative bacteria (GNB) are among the most frequent complications in the immunocompromised cancer patients because of their considerable morbidity and mortality. Therefore, the aim of the current study was to characterize the prevalence of carbapenemase-producing GNB recovered from febrile neutropenic pediatric cancer patients in Egypt. Standard methods were used for identification, sensitivity testing (Kirby-Bauer and broth microdilution method according to CLSI guidelines). Standard methods were applied for both phenotypic and genotypic detection of the carbapenemase-producing GNB. A total of 185 GNB were recovered from different clinical specimens, Escherichia (E.) coli (86; 46.48%), followed by Klebsiella spp. (71; 38.37%), Acinetobacter (A.) baumannii (7; 3.78%) and others including Pseudomonas spp., Enterobacter (Ent.) cloacae and Proteus spp. (21; 11.35%). It is a matter of concern that 116 out of 171 enterobacterial isolates (94.15%) showed resistance to three or more antimicrobial classes and were considered multidrug resistant. Additionally, the rate of carbapenem-resistance displayed a worrisome trend as 113 out of 171 enterobacterial isolates (66.08%) and 12 out of 14 non fe...Continue Reading

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Citations

Mar 7, 2020·Future Science OA·Ann A Elshamy, Khaled M Aboshanab
Nov 13, 2020·Infectious Diseases and Therapy·Reham WasfiOla A Abd El-Rahman
Feb 10, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·May SherifAmani El-Kholy
Jun 18, 2019·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Carole Ayoub MoubareckDolla Karam Sarkis
Jun 4, 2021·Infection and Drug Resistance·Amani El-KholyRamy Kotb
Jul 2, 2021·BioMed Research International·Saba Gebremichael TekeleSamuel Kinde Birru

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Methods Mentioned

BETA
PCR

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