Phenotypic suppression in Escherichia coli by chloramphenicol and other reversible inhibitors of the ribosome.

Journal of Bacteriology
C Kirschmann, B D Davis

Abstract

Antibiotics that interfere reversibly with various aspects of ribosomal function (chloramphenicol, tetracycline, erythromycin, and spectinomycin) are shown to antagonize, at barely inhibitory concentrations, the inhibitory effect of low concentrations of streptomycin (SM) on the growth of Escherichia coli. Paradoxically, these compounds can also replace SM in supporting the growth of conditionally SM-dependent mutants. Chloramphenicol produced about as much phenotypic suppression as SM in SM-sensitive strains, but less than that attainable with high concentrations of SM in resistant strains. The antagonism to SM inhibition and the phenotypic suppression appear to be specific for those growth inhibitors that act on the ribosome. Since inhibitors of the 50S subunit of the ribosome (chloramphenicol, erythromycin) are as active as inhibitors of the 30S subunit, it is suggested that phenotypic suppression by borderline concentrations of ribosome inhibitors does not necessarily depend on an alteration of the recognition region of the ribosome. Alternatively, partial inhibition of the ribosomes might change the environment in a way that would influence the frequency of misreading. Phenotypic suppression by a low concentration of SM as...Continue Reading

References

Oct 14, 1967·Journal of Molecular Biology·P AndersonB D Davis
Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·L GoriniL Breckenridge
Apr 22, 1964·Biochemical and Biophysical Research Communications·D Vazquez
Nov 28, 1967·Journal of Molecular Biology·E Cundliffe, K McQuillen
Sep 3, 1965·Science·J DaviesB D Davis
Jan 1, 1967·Journal of Bacteriology·D H Smith, B D Davis
Jun 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·I SuzukaA Kaji
Apr 1, 1962·Journal of Bacteriology·P H PLOTZ, B D DAVIS
Dec 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·P S SYPHERD, N STRAUSS
Apr 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·J E DAVIES
Mar 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·L GORINI, E KATAJA
May 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·J DAVIESL GORINI
Nov 6, 1964·Science·J R WHITE, H L WHITE
Oct 1, 1964·Journal of Molecular Biology·R R TRAUT, R E MONRO
Mar 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·M HIEROWSKI
Jun 18, 1965·Biochemical and Biophysical Research Communications·R H CONNAMACHER, H G MANDEL
Jul 1, 1950·Journal of Bacteriology·B D DAVIS, E S MINGIOLI

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Citations

Oct 25, 1969·Nature·J Modolell, B D Davis
Apr 17, 1978·Molecular & General Genetics : MGG·V KuhrováJ Reich
Mar 25, 2021·Nature Communications·Ingo WohlgemuthMarina V Rodnina
Aug 1, 2009·EcoSal Plus·Steven T Gregory
Nov 1, 1969·Journal of Bacteriology·P Anderson

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