Phenotypical analysis of adult rat olfactory ensheathing cells on 3-D collagen scaffolds

Neuroscience Letters
Bin WangJianwu Dai

Abstract

Olfactory ensheathing cell (OEC) transplantation is a promising or potential therapy for spinal cord injury (SCI). However, the effects of injecting OECs directly into SCI site have been limited and unsatisfied due to the complexity of SCI. To improve the outcome, proper biomaterials are thought to be helpful since these materials would allow the cells to grow three-dimensionally and guide cell migration. In this paper, we have studied the behavior of OECs in two-dimensional (2-D) condition as well as on three-dimensional (3-D) collagen scaffolds by analyzing their phenotypes such as cell proliferation, apoptosis, morphology, and gene activities of some neurotrophic factors and myelin proteins. OECs proliferation rate was increased on 3-D collagen scaffolds compared to the 2-D culture condition. OECs on 3-D collagen scaffolds also showed less apoptosis. In addition, OECs on 3-D collagen scaffolds maintained the original spindle-shape morphology and P75NTR gene activity. NGF, BDNF, and PLP were found to be upregulated in OECs cultured on 3-D collagen scaffolds by the semi-quantitative RT-PCR approach. The results suggested that 3-D collagen scaffolds provide suitable environments for the OECs to maintain their morphology as well...Continue Reading

References

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Citations

Jul 25, 2012·Cell Biochemistry and Biophysics·Manuel Pérez-GarnésManuel Monleón Pradas
May 26, 2012·Nanomedicine·Jacqueline Li-Ling KuehRobert Stevens
Jun 19, 2007·Neuroscience Letters·Yanhong ZhaoJianwu Dai
May 20, 2015·Neuroscience Letters·Cristina Martínez-RamosManuel Monleón Pradas
Oct 25, 2013·Cell Transplantation·Zhihai FanHuanxiang Zhang
Dec 5, 2018·Macromolecular Bioscience·Christopher F AdamsDivya M Chari
Jan 11, 2020·Integrative Biology : Quantitative Biosciences From Nano to Macro·Michael G EvansDivya M Chari
Jul 5, 2016·Bioengineering & Translational Medicine·Nilesh GuptaVivek Gupta
Feb 15, 2020·Journal of Neurotrauma·Aaron D GilmourJames A St John
Jan 30, 2018·Frontiers in Cellular Neuroscience·Shengwen LiuRadhika Puttagunta
Oct 13, 2020·Journal of Neural Engineering·Martina BrofigaPaolo Massobrio

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