Phenotypical and functional characterization of intrahepatic bile duct cells from common duct ligated mice

The Journal of Laboratory and Clinical Medicine
W HuH L Sharp

Abstract

Bile duct cells (BDCs), especially intrahepatic cholangiocytes, are primary targets of immune-related injury in such pathologic processes as liver graft rejection, liver graft-versus-host disease, and primary sclerosing cholangitis. Cholestasis and progressive loss of intrahepatic BDCs indicate chronicity in these diseases. The present investigation characterizes the acquisition of immune markers of intrahepatic BDCs isolated from mice after bile duct ligation. Purified BDCs from cholestatic C57BL/6 (H-2b) mice express not only the major histocompatibility complex (MHC) class I and class II antigens but also the costimulatory molecules B7-1 (CD80) and B7-2 (CD86). The expression of the MHC class II molecules on BDCs before and after interferon (IFN)-gamma exposure is also demonstrated by immunohistochemistry on the cultured cells. Cytotoxicity assays indicate the vulnerability of these cells as targets for immunologic injuries. Effector cells generated from B10.BR splenocytes (H-2k) against C57BL/6 splenocytes (H-2b) show comparable killing of BDCs and EL4 cells, the latter being a lymphoma line that was established from the C57BL/6N (H-2b) mouse. An immortalized mouse BDC line (IBDC) is included in this study to validate some ...Continue Reading

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Citations

Jul 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G HrehaJ M Vierling
Jul 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S Reynoso-PazM E Gershwin
Apr 29, 1998·Mayo Clinic Proceedings·A J Demetris
Sep 11, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gaurav SyalJonathan A Dranoff
Jan 30, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Guoku HuXian-Ming Chen
Sep 20, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Celine S LagesAlexander G Miethke
Dec 1, 1996·The Journal of Laboratory and Clinical Medicine·D H Adams
Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C D HowellW Chen

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