Phenotypical characterization, distribution and quantification of different mast cell subtypes in transmural biopsies from the gastrointestinal tract of cats with inflammatory bowel disease

Veterinary Immunology and Immunopathology
Sven KleinschmidtMarion Hewicker-Trautwein

Abstract

In this study subtypes, distribution and number of mast cells were investigated within mucosa and submucosa of the gastrointestinal tract of 24 cats with inflammatory bowel disease (IBD) in comparison to 11 control cats. Paraffin sections of formalin-fixed transmural gastrointestinal biopsies from stomach, duodenum, jejunum, ileum and colon were examined. Mast cells were phenotyped and quantified based on their chymase and tryptase content, by applying a combined enzyme-histochemical and immunohistochemical double-labeling technique and on their heparin content by a metachromatic staining method (kresylecht-violet, MC(KEV)). Mast cells containing both chymase and tryptase were not found in any of the samples examined. Furthermore, in the stomach neither chymase (MC(C)) nor tryptase (MC(T)) bearing mast cells were detected. In cats with lymphocytic-plasmacytic enteritis or enterocolitis elevated numbers of MC(T) or MC(C) were identified in comparison to controls mainly located in the inflamed segments. The highest quantity of MC(C) was found in cats with eosinophilic gastroenterocolitis or enterocolitis in comparison to other IBD forms, but only minor numbers of MC(T) were detected in these cases. In cats with fibrosing enteropa...Continue Reading

References

Jan 1, 1987·International Archives of Allergy and Applied Immunology·Y KitamuraT Nakano
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Citations

Dec 18, 2015·Veterinary Immunology and Immunopathology·Romy M Heilmann, Jan S Suchodolski
Jun 16, 2011·Anatomia, histologia, embryologia·S KleinschmidtM Hewicker-Trautwein
Jun 30, 2010·Journal of Veterinary Internal Medicine·A E JergensC Wang
Feb 26, 2020·Histochemistry and Cell Biology·Agnese StanzaniRoberto Chiocchetti

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