Phenotyping Circadian Rhythms in Mice

Current Protocols in Mouse Biology
Kristin Eckel-Mahan, Paolo Sassone-Corsi

Abstract

Circadian rhythms take place with a periodicity of 24 hr, temporally following the rotation of the earth around its axis. Examples of circadian rhythms are the sleep/wake cycle, feeding, and hormone secretion. Light powerfully entrains the mammalian clock and assists in keeping animals synchronized to the 24-hour cycle of the earth by activating specific neurons in the "central pacemaker" of the brain, the suprachiasmatic nucleus. Absolute periodicity of an animal can deviate slightly from 24 hr as manifest when an animal is placed into constant dark or "free-running" conditions. Simple measurements of an organism's activity in free-running conditions reveal its intrinsic circadian period. Mice are a particularly useful model for studying circadian rhythmicity due to the ease of genetic manipulation, thus identifying molecular contributors to rhythmicity. Furthermore, their small size allows for monitoring locomotion or activity in their homecage environment with relative ease. Several tasks commonly used to analyze circadian periodicity and plasticity in mice are presented here including the process of entrainment, determination of tau (period length) in free-running conditions, determination of circadian periodicity in respon...Continue Reading

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Citations

Oct 14, 2016·Chronobiology International·Lisa A GottliebMorten B Thomsen
May 18, 2019·British Journal of Pharmacology·Laetitia S GasparCláudia Cavadas
Aug 3, 2019·PloS One·Surjeet SinghMajid H Mohajerani
Sep 1, 2020·Current Protocols in Mouse Biology·Laurence A BrownStuart N Peirson
Aug 23, 2020·Brain Sciences·Patricia Sampedro-PiqueroLuis Javier Santin
May 11, 2019·International Journal of Molecular Sciences·Ellen PaatelaNobuaki Kikyo
Jan 6, 2019·Molecular Psychiatry·Galen MissigWilliam A Carlezon
Nov 27, 2020·Frontiers in Neuroscience·John Samuel BassVaishnav Krishnan
Apr 24, 2021·Clinical Psychopharmacology and Neuroscience : the Official Scientific Journal of the Korean College of Neuropsychopharmacology·Jeonghyun ParkJung Hyun Lee
May 27, 2021·STAR Protocols·Thomas MortimerKevin B Koronowski
Jun 28, 2021·Acta Physiologica·Lisa A GottliebMorten B Thomsen
Aug 5, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yuval IshayYaron Ilan
May 14, 2021··Kevin B. KoronowskiSalvador Aznar Benitah

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